Fungus/Mushroom Resource and Research Center, Faculty of Agriculture, Tottori University, 4-101 Minami, Koyama, Tottori 680-8553, Japan.
Mycologia. 2013 Sep-Oct;105(5):1110-25. doi: 10.3852/12-147. Epub 2012 Sep 6.
We analyzed the DNA sequences of four gene regions, 28S and 18S rDNA, the ITS region and rpb2, to obtain a high resolution phylogenetic tree of Dacrymycetes. In addition, we comparatively studied micro- and macromorphological characteristics of representative species. The traditional generic classification based on morphological characteristics was not reflected by our molecular phylogenies. Ancestral state reconstructions indicated that the morphology of basidia and clamp connections are evolutionarily stable. In contrast, basidiocarps and basidiospore septation patterns appear variable. Dacrymyces unisporus shares the dolipores with non-perforate parenthesomes typical of other dacrymycetous taxa but is a unique species having predominantly non-bifurcate basidia and subglobose to ovoid basidiospores with transverse and longitudinal septa. In molecular phylogenies this species is a member of Dacrymycetes but always occupies a sister position in relation to the rest of the Dacrymycetes. Based on our results we propose a new genus, Unilacryma, for D. unisporus. For proper accommodation of this taxon, we introduce the family Unilacrymaceae and the order Unilacrymales.
我们分析了四个基因区域的 DNA 序列，即 28S 和 18S rDNA、ITS 区和 rpb2，以获得高度分辨率的伞菌目系统发育树。此外，我们还比较研究了代表物种的微观和宏观形态特征。基于形态特征的传统属分类与我们的分子系统发育不符。祖先状态重建表明，担子和连接扣的形态在进化上是稳定的。相比之下，担子果和担子孢子的分隔模式似乎是可变的。栓菌属unisporus 与非穿孔的假囊体共享多管孔，这是其他伞菌目类群的典型特征，但它是一个独特的物种，主要具有不分叉的担子和近球形到卵形的担子孢子，具有横向和纵向的分隔。在分子系统发育中，这个物种是伞菌目的成员，但总是与伞菌目的其他成员处于姐妹关系。基于我们的结果，我们提出了一个新属，Unilacryma，用于 D. unisporus。为了正确容纳这个分类群，我们引入了 Unilacrymaceae 科和 Unilacrymales 目。
