Engineering Faculty, Department of Chemical Engineering, Istanbul University, 34320 Avcilar, Istanbul, Turkey.
Ultrason Sonochem. 2013 Jan;20(1):595-602. doi: 10.1016/j.ultsonch.2012.07.029. Epub 2012 Aug 11.
In the present article, ultrasound-assisted extraction (UAE) of polyphenols from agricultural and industrial waste of olive oil and table oil productions, olive tree (Olea europaea) leaves were investigated. The aim of the study is to examine the extraction parameters such as solvent concentration (0-100% ethanol (EtOH), v/v), the ratio of solid to solvent (25-50mg/mL) and extraction time (20-60 min), and to obtain the best possible combinations of these parameters through response surface methodology (RSM). The extract yield was stated as mg extract per g of dried leaf (DL). Total phenolic content was expressed in gallic acid equivalent (GAE) per g of dried leaf. Free radical scavenging activity for the antioxidant capacity was tested by 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical. The second order polynomial model gave a satisfactory description of the experimental data. 201.2158 mg extract/g DL, 25.0626 mg GAE/g DL, and 95.5610% in respect to inhibition of DPPH radical were predicted at the optimum operating conditions (500 mg solid to 10 mL solvent ratio, 60 min of extraction time and 50% EtOH composition), respectively.
本文研究了超声辅助提取（UAE）橄榄和植物油生产过程中的农业和工业废物、橄榄树（Olea europaea）叶片中的多酚。研究目的是通过响应面法（RSM）研究提取参数，如溶剂浓度（0-100%乙醇（EtOH），v/v）、固液比（25-50mg/mL）和提取时间（20-60min），并获得这些参数的最佳组合。提取物产率表示为每克干叶（DL）的提取物毫克数。总酚含量以每克干叶的没食子酸当量（GAE）表示。通过 1,1-二苯基-2-苦基肼（DPPH）自由基测定法测试了抗氧化能力的自由基清除活性。二阶多项式模型对实验数据进行了很好的描述。在最佳操作条件下（500mg 固体与 10mL 溶剂比、60min 的提取时间和 50%EtOH 组成），分别预测出提取物产率为 201.2158mg/gDL、25.0626mgGAE/gDL 和 95.5610%抑制 DPPH 自由基。
