School of Chemistry (Building F11), The University of Sydney, NSW, 2006, Australia.
Chem Asian J. 2012 Nov;7(11):2621-8. doi: 10.1002/asia.201200627. Epub 2012 Sep 10.
The anion binding ability of a family of bis(Zn(II)-Dpa) functionalized cyclic peptides has been investigated using displacement assays with a fluorescent coumarin indicator in water, saline solution, and Krebs buffer. Non-binding side-chain steric bulk, the relative position of binding sites, and the scaffold size were all found to affect the ability of these receptors to discriminate between polyphosphate ions. Most receptors showed some selectivity for pyrophosphate over ATP and ADP in water and saline, and this selectivity was significantly enhanced in the biologically relevant Krebs buffer giving chemosensing ensembles capable of selective recognition of pyrophosphate in the presence of excess ATP.
采用含有荧光香豆素指示剂的置换实验，研究了一系列双（Zn（II）-Dpa）功能化环肽对阴离子的结合能力。在水、盐溶液和 Krebs 缓冲液中，非结合侧链的空间位阻、结合位点的相对位置和支架大小均影响这些受体区分多磷酸盐离子的能力。大多数受体在水和盐溶液中对焦磷酸盐相对于 ATP 和 ADP 显示出一定的选择性，而在具有生物学相关性的 Krebs 缓冲液中，这种选择性得到了显著增强，形成了能够在存在过量 ATP 的情况下选择性识别焦磷酸盐的化学感应组合体。
