Department of Applied Chemistry, Graduate School of Engineering, The University of Tokyo, Bunkyo, Tokyo, Japan.
Electrophoresis. 2012 Sep;33(17):2668-73. doi: 10.1002/elps.201200099.
The mixing of fluids using AC electrothermal flow (AC-ETF) is presented. A pair of coplanar electrodes with a sinusoidal interelectrode gap was used to enhance the mixing in a microchannel. To demonstrate the performance of the mixer, conventional dilution experiments were conducted using Texas Red-labeled dextran. The dependence of mixing on the salt concentration (10(-3) ∼ 10(-1) mol dm(-3) ) of the solutions and frequency (100 kHz ∼ 5 MHz) of the applied voltage were investigated. AC-ETF was responsible for the mixing at salt concentrations >10(-2) mol dm(-3) , whereas the effect of AC-EOF was suggested to play a role at concentrations <10(-2) mol dm(-3) in the low-frequency region. The fluorogenic reaction of human serum albumin (HSA) with SYPRO Red in the mixer was also examined, and results showed that enrichment of fluorescence intensity and an almost uniform distribution of stained HSA were achieved. The present mixer can be employed as a powerful tool to facilitate efficient chemical and biomedical analysis on microfluidic devices.
本文提出了一种利用交流电热流动（AC-ETF）混合流体的方法。使用一对具有正弦相间电极间隙的共面电极来增强微通道中的混合。为了演示混合器的性能，使用 Texas Red 标记的葡聚糖进行了常规稀释实验。研究了混合对溶液盐浓度（10(-3) ∼ 10(-1) mol dm(-3) ）和施加电压频率（100 kHz ∼ 5 MHz）的依赖性。在盐浓度>10(-2) mol dm(-3)时，AC-ETF 负责混合，而在低频率区域浓度<10(-2) mol dm(-3)时，建议 AC-EOF 发挥作用。还检查了混合器中人血清白蛋白（HSA）与 SYPRO Red 的荧光反应，结果表明实现了荧光强度的富集和染色 HSA 的几乎均匀分布。本混合器可用作在微流控设备上进行高效化学和生物医学分析的有力工具。
