Sibley School of Mechanical and Aerospace Engineering, Cornell University, Ithaca, NY, USA.
Biomed Microdevices. 2013 Feb;15(1):97-108. doi: 10.1007/s10544-012-9691-2.
We present the design, analysis, construction, and culture results of a microfluidic device for the segregation and chemical stimulation of primary rat hippocampal neurons. Our device is designed to achieve spatio-temporal solute delivery to discrete sections of neurons with mitigated mechanical stress. We implement a geometric guidance technique to direct axonal processes of the neurons into specific areas of the device to achieve solute segregation along routed cells. Using physicochemical modeling, we predict flows, concentration profiles, and mechanical stresses within pertiment sections of the device. We demonstrate cell viability and growth within the closed device over a period of 11 days. Additionally, our modeling methodology may be generalized and applied to other device geometries.
我们介绍了一种用于分离和化学刺激原代大鼠海马神经元的微流控装置的设计、分析、构建和培养结果。我们的装置旨在实现对神经元离散部分的时空溶质输送，同时减轻机械应力。我们采用几何引导技术将神经元的轴突过程引导到装置的特定区域，以实现沿路由细胞的溶质分离。通过物理化学建模，我们预测了装置中特定区域的流动、浓度分布和机械应力。我们证明了在 11 天的时间内，细胞在封闭装置内的存活和生长。此外，我们的建模方法可以推广并应用于其他装置几何形状。
