Wang Jinyi, Ren Li, Li Li, Liu Wenming, Zhou Jing, Yu Wenhao, Tong Denwen, Chen Shulin
College of Animal Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China.
Lab Chip. 2009 Mar 7;9(5):644-52. doi: 10.1039/b813495b. Epub 2008 Dec 18.
Recently, microfluidic systems have shown great potential in the study of molecular and cellular biology. With its excellent properties, such as miniaturization, integration and automation, to name just a few, microfluidics creates new opportunities for the spatial and temporal control of cell growth and environmental stimuli in vitro. In the field of neuroscience, microfluidic devices offer precise control of the microenvironment surrounding individual cells, and the delivery of biochemical or physical cues to neural networks or single neurons. The intent of this review is to outline recent advances in microfluidic-based applications in neurobiology, with emphasis on neuron culture, neuron manipulation, neural stem cell differentiation, neuropharmacology, neuroelectrophysiology, and neuron biosensors. It also aims to stimulate development of microfluidic-based applications in neurobiology by involving scientists from various disciplines, especially neurobiology and microtechnology.
近年来，微流控系统在分子和细胞生物学研究中展现出了巨大潜力。微流控技术具有诸多优异特性，如小型化、集成化和自动化等，为体外细胞生长及环境刺激的时空控制创造了新机遇。在神经科学领域，微流控装置能够精确控制单个细胞周围的微环境，并向神经网络或单个神经元传递生化或物理信号。本综述旨在概述基于微流控技术在神经生物学应用中的最新进展，重点关注神经元培养、神经元操控、神经干细胞分化、神经药理学、神经电生理学以及神经元生物传感器。同时，本综述还旨在通过吸引来自不同学科，特别是神经生物学和微技术领域的科学家，推动基于微流控技术在神经生物学中的应用发展。
