Caprioli R M, Lin S N
Department of Biochemistry and Molecular Biology, University of Texas Medical School, Houston 77030.
Proc Natl Acad Sci U S A. 1990 Jan;87(1):240-3. doi: 10.1073/pnas.87.1.240.
The combination of microdialysis and fast-atom bombardment mass spectrometry has been used to follow the pharmacokinetics of penicillin G directly in the blood-stream of a live rat. After the intramuscular injection of the antibiotic, the blood dialysate was allowed to flow into the mass spectrometer via the continuous-flow/fast-atom bombardment interface. Tandem mass spectrometry provided the means for isolating and recording the ion fragments produced from the drug as the dialysate was exposed to the ionization process. The pharmacokinetic results obtained are compared with those previously reported in the literature, and this on-line method is discussed in terms of its particular advantages.
微透析与快原子轰击质谱联用技术已被用于直接跟踪活鼠血流中青霉素G的药代动力学。肌肉注射抗生素后，血液透析液通过连续流动/快原子轰击接口流入质谱仪。串联质谱提供了一种手段，用于在透析液暴露于电离过程时分离并记录药物产生的离子碎片。将所获得的药代动力学结果与文献中先前报道的结果进行比较，并就这种在线方法的特殊优势进行了讨论。
