Caprioli R M
Department of Biochemistry and Molecular Biology, University of Texas Medical School, Houston 77030.
Biomed Environ Mass Spectrom. 1988 Oct;16(1-12):35-9. doi: 10.1002/bms.1200160107.
The continuous-flow fast atom bombardment probe interface has been used for the direct analysis of aqueous biochemical reactions. This device was used in two ways: in a constant flow mode and a flow injection mode. Each method has particular advantages which are illustrated with specific applications, including measurement of enzyme kinetics, comparative rates of hydrolysis of multi-substrate/single enzyme reactions, C-terminal peptide sequence analysis, and characterization of multi-enzyme/single substrate systems.
连续流快原子轰击探针接口已用于水相生化反应的直接分析。该装置有两种使用方式：恒流模式和流动注射模式。每种方法都有其独特的优势，并通过具体应用加以说明，包括酶动力学测量、多底物/单酶反应水解速率的比较、C端肽序列分析以及多酶/单底物系统的表征。
