Selling B H, Allison R F, Kaesberg P
Institute for Molecular Virology, University of Wisconsin, Madison 53706.
Proc Natl Acad Sci U S A. 1990 Jan;87(1):434-8. doi: 10.1073/pnas.87.1.434.
Newly synthesized virions of flock house virus (FHV), an insect nodavirus, were detected in plant cells inoculated with FHV RNA. FHV was found in whole plants of barley (Hordeum vulgare), cowpea (Vigna sinensis), chenopodium (Chenopodium hybridum), tobacco (Nicotiana tabacum), and Nicotiana benthamiana and in protoplasts derived from barley leaves. Virions produced in plants contained newly synthesized RNA as well as newly synthesized capsid protein. These results show that the intracellular environment in these plants is suitable for synthesis of a virus normally indigenous only to insects. Such synthesis involves, minimally, translation of viral RNA, RNA replication, and virion assembly. Inoculation of barley protoplasts with FHV virions resulted in synthesis of small amounts of progeny virions, suggesting that FHV virions are capable of releasing their RNA in plant cells. In N. benthamiana, virions resulting from inoculation with RNA were detected not only in inoculated leaves but also in other leaves of inoculated plants, suggesting that virions could move in this plant species. Such movement probably occurs by a passive transport through the vascular system rather than by an active transport involving mechanisms that have evolved for plant viruses.
在接种了禽呼肠孤病毒（FHV，一种昆虫诺达病毒）RNA的植物细胞中检测到了新合成的FHV病毒粒子。在大麦（Hordeum vulgare）、豇豆（Vigna sinensis）、藜（Chenopodium hybridum）、烟草（Nicotiana tabacum）和本氏烟草（Nicotiana benthamiana）的整株植物以及从大麦叶片获得的原生质体中都发现了FHV。植物中产生的病毒粒子含有新合成的RNA以及新合成的衣壳蛋白。这些结果表明，这些植物的细胞内环境适合于合成一种通常仅在昆虫中天然存在的病毒。这种合成至少涉及病毒RNA的翻译、RNA复制和病毒粒子组装。用FHV病毒粒子接种大麦原生质体导致少量子代病毒粒子的合成，这表明FHV病毒粒子能够在植物细胞中释放其RNA。在本氏烟草中，接种RNA后产生的病毒粒子不仅在接种叶片中被检测到，而且在接种植物的其他叶片中也被检测到，这表明病毒粒子可以在这种植物中移动。这种移动可能是通过维管系统的被动运输发生的，而不是通过涉及为植物病毒进化出的机制的主动运输。
