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一个新的基因 ardD 决定了非接合转座子 Tn5053 的反限制活性，并且位于 tniA 基因的反义位置。

A novel gene, ardD, determines antirestriction activity of the non-conjugative transposon Tn5053 and is located antisense within the tniA gene.

机构信息

State Research Institute of Genetics and Selection of Industrial Microorganisms (GosNIIgenetika), Moscow, Russia.

出版信息

FEMS Microbiol Lett. 2012 Dec;337(1):55-60. doi: 10.1111/1574-6968.12005. Epub 2012 Oct 3.

DOI:10.1111/1574-6968.12005
PMID:22967207
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3533173/
Abstract

The mercury-resistance transposon Tn5053 inhibits restriction activity of the type I restriction-modification endonuclease EcoKI in Escherichia coli K12 cells. This is the first report of antirestriction activity of a non-conjugative transposon. The gene (ardD) coding for the antirestriction protein has been cloned. The ardD gene is located within the tniA gene, coding for transposase, on the complementary strand. The direction of transcription is opposite to transcription of the tniA gene.

摘要

汞抗性转座子 Tn5053 抑制大肠杆菌 K12 细胞中 I 型限制修饰内切酶 EcoKI 的限制活性。这是第一个报道非接合性转座子具有反限制活性的报告。编码反限制蛋白的基因 (ardD) 已经被克隆。ardD 基因位于编码转座酶的 tniA 基因的互补链上。转录方向与 tniA 基因的转录方向相反。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2683/3533173/97fe6e94a888/fml0337-0055-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2683/3533173/e7483c9b7dce/fml0337-0055-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2683/3533173/fe51e4657950/fml0337-0055-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2683/3533173/97fe6e94a888/fml0337-0055-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2683/3533173/e7483c9b7dce/fml0337-0055-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2683/3533173/fe51e4657950/fml0337-0055-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2683/3533173/97fe6e94a888/fml0337-0055-f3.jpg

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