Lanchuk Yulia V, Ulasevich Sviatlana A, Fedotova Tatiana A, Kolpashchikov Dmitry M, Skorb Ekaterina V
ITMO University Lomonosova St. 9 191002 St. Petersburg Russian Federation
Chemistry Department University of Central Florida Orlando FL USA.
RSC Adv. 2018 Nov 9;8(66):37735-37739. doi: 10.1039/c8ra06711b. eCollection 2018 Nov 7.
Blind and color blind people cannot use colorimetric diagnostics; the problem is especially severe in rural areas where high temperatures and the absence of electricity challenge modern diagnostics. Here we propose to replace the unstable component of a diagnostic test, HO, with stable TiO. Under UV irradiation, TiO forms reactive oxygen species that initiate polymerization of acrylamide causing liquid-to-gel transition in an analyte-dependent manner. We demonstrate that specific DNA sequences can be detected using this approach. This development may enable the detection of biological molecules by users with limited resources, for example in developing countries or for travelers in remote areas.
盲人及色盲患者无法使用比色诊断法；在农村地区，这一问题尤为严重，因为高温和电力短缺对现代诊断技术构成了挑战。在此，我们提议用稳定的二氧化钛（TiO）替代诊断测试中不稳定的成分——过氧化氢（HO）。在紫外线照射下，二氧化钛会形成活性氧物种，引发丙烯酰胺聚合，从而以依赖分析物的方式导致液体向凝胶转变。我们证明了使用这种方法可以检测特定的DNA序列。这一进展可能使资源有限的用户能够检测生物分子，例如在发展中国家或偏远地区的旅行者。
