Department of Pharmaceutical Analysis, School of Pharmacy, Fujian Medical University, Fuzhou 350004, China.
Biosens Bioelectron. 2013 May 15;43:1-5. doi: 10.1016/j.bios.2012.11.031. Epub 2012 Dec 6.
A chemiluminescent cholesterol sensor with good selectivity and enhanced sensitivity was constructed based upon the peroxidase-like activity of cupric oxide nanoparticles. Cupric oxide nanoparticles can catalyze the oxidation of luminol by H2O2, which was produced by the reaction of cholesterol and oxygen that was catalyzed by cholesterol oxidase. Therefore, the oxidation of cholesterol could be transduced into the chemiluminescence of luminol by combining these two reactions. Under the optimum conditions, the CL intensity was proportional to the concentration of cholesterol over the range of 0.625-12.5μM and a detection limit was 0.17μM. The applicability of proposed method has been validated by determination of cholesterol in milk powder and human serum samples with satisfactory results.
基于氧化铜纳米粒子的过氧化物酶样活性，构建了一种对胆固醇具有良好选择性和增强灵敏度的化学发光胆固醇传感器。氧化铜纳米粒子可以催化胆固醇和胆固醇氧化酶催化的氧气反应生成的 H2O2 氧化鲁米诺。因此，通过结合这两个反应，胆固醇的氧化可以转化为鲁米诺的化学发光。在最佳条件下，CL 强度与胆固醇的浓度在 0.625-12.5μM 范围内呈正比，检测限为 0.17μM。该方法已通过测定奶粉和人血清样品中的胆固醇得到验证，结果令人满意。
