Key Laboratory of Photochemical Biomaterials and Energy Storage Materials, Heilongjiang Province, College of Chemistry and Chemical Engineering, Harbin Normal University, Harbin, 150025, People's Republic of China.
Mikrochim Acta. 2019 Oct 8;186(11):686. doi: 10.1007/s00604-019-3801-2.
The authors describe boronate-modified hollow dummy template imprinted polymers (B-hDIPs) for selective and sensitive extraction of the catecholamines norepinephrine (NE), epinephrine (E), and dopamine (DA) from urine samples prior to their detection by HPLC with UV detector. The B-hDIPs were prepared by using (a) nano-TiO as the sacrificial support, (b) 3-carboxyphenylboronic acid (CPBA) as the boronate-affinity functional monomer, and (c) catechol which acts as dummy template. The nano-TiO can be etched off by using hydrofluoric acid to form the hollow imprint. The main advantages of using such hollow polymers arise from their controllable hole structure which favors template removal and fast mass transfer. The B-hDIPs material was characterized by FT-IR, transmission electron microscope, dynamic light scattering, X-ray photoelectron spectrum, and thermal gravimetric analysis. The effects of imprinting conditions, sample pH, sample flow rate, adsorbent amount, selectivity and competitive capacities were investigated. Under optimal conditions, the limits of detection for the catecholamines are in the range from 15 to 47 ng mL. The method was successfully applied to their determination of NE, E, and DA in spiked urine, with recoveries ranging from 63.4 to 106.2%. Graphical abstract Schematic presentation of boronate-modified hollow dummy template imprinted polymers as an adsorbent to extraction of the catecholamines (norepinephrine, epinephrine, and dopamine) from urine samples prior to their detection by HPLC-UV.
作者描述了硼酸盐修饰的空心假模板印迹聚合物（B-hDIPs），用于选择性和灵敏地从尿液样品中提取儿茶酚胺去甲肾上腺素（NE）、肾上腺素（E）和多巴胺（DA），然后通过 HPLC-UV 进行检测。B-hDIPs 的制备方法如下：（a）纳米 TiO 作为牺牲支撑，（b）3-羧基苯硼酸（CPBA）作为硼酸盐亲和性功能单体，（c）儿茶酚作为假模板。可以使用氢氟酸蚀刻掉纳米 TiO 以形成空心印迹。使用这种空心聚合物的主要优点在于其可控的孔结构有利于模板去除和快速传质。B-hDIPs 材料通过傅里叶变换红外光谱、透射电子显微镜、动态光散射、X 射线光电子能谱和热重分析进行了表征。考察了印迹条件、样品 pH 值、样品流速、吸附剂用量、选择性和竞争能力的影响。在最佳条件下，儿茶酚胺的检测限范围为 15-47 ng mL。该方法成功应用于尿液中 NE、E 和 DA 的测定，回收率在 63.4-106.2%之间。
