Rukayadi Yaya, Hwang Jae-Kwan
Department of Biotechnology & Bioproducts Research Center, Yonsei University, Seoul 120-749, Korea.
J Basic Microbiol. 2006;46(5):410-5. doi: 10.1002/jobm.200510088.
Colonization on the surface of tooth by Streptococcus mutans is an important step in the initiation of dental plaque. Polystyrene microtiter plates have been employed to study bacterial colonization and biofilm formation of periodontal bacteria. The objective of this work was to evaluate the effect of coating the wells of a polystyrene microtiter plate with xanthorrhizol isolated from java turmeric (Curcuma xanthorrhiza Roxb.) on Strep. mutans biofilm formation. Our studies demonstrated that coating of a polystyrene microtiter plate with 5 microg/ml of xanthorrhizol resulted in significant (up to 60%) reduction of adherent cells compared to that of cells in uncoated wells. This result suggests that xanthorrhizol displays potent activity in preventing Strep. mutans biofilm formation.
变形链球菌在牙齿表面的定植是牙菌斑形成起始过程中的重要一步。聚苯乙烯微量滴定板已被用于研究牙周细菌的细菌定植和生物膜形成。这项工作的目的是评估用从爪哇姜黄（Curcuma xanthorrhiza Roxb.）中分离出的姜黄醇对聚苯乙烯微量滴定板孔进行包被对变形链球菌生物膜形成的影响。我们的研究表明，与未包被孔中的细胞相比，用5微克/毫升姜黄醇包被聚苯乙烯微量滴定板会使贴壁细胞显著减少（高达60%）。这一结果表明，姜黄醇在预防变形链球菌生物膜形成方面具有强大的活性。
