Department of Oncology and Radiotherapy, University of Turku and Turku University Hospital, FIN-20521 Turku, Finland.
World J Gastroenterol. 2012 Sep 7;18(33):4549-56. doi: 10.3748/wjg.v18.i33.4549.
To investigate the prognostic value of CD44 variant 6 (CD44v6), a membranous adhesion molecule, in rectal cancer.
Altogether, 210 rectal cancer samples from 214 patients treated with short-course radiotherapy (RT, n = 90), long-course (chemo) RT (n = 53) or surgery alone (n = 71) were studied with immunohistochemistry for CD44v6. The extent and intensity of membranous and cytoplasmic CD44v6 staining, and the intratumoral membranous staining pattern, were analyzed.
Membranous CD44v6 expression was seen in 84% and cytoplasmic expression in 81% of the cases. In 59% of the tumors with membranous CD44v6 expression, the staining pattern in the invasive front was determined as "front-positive" and in 41% as "front-negative". The latter pattern was associated with narrower circumferential margin (P = 0.01), infiltrative growth pattern (P < 0.001), and shorter disease-free survival in univariate survival analysis (P = 0.022) when compared to the "front-positive" tumors.
The lack of membranous CD44v6 in the rectal cancer invasive front could be used as a method to identify patients at increased risk for recurrent disease.
研究膜黏附分子 CD44 变体 6（CD44v6）在直肠癌中的预后价值。
对 214 例接受短程放疗（RT，n=90）、长程（化疗）RT（n=53）或单纯手术（n=71）治疗的直肠癌患者的 210 例直肠癌样本进行了 CD44v6 的免疫组织化学研究。分析了膜和细胞质 CD44v6 染色的程度和强度，以及肿瘤内膜染色模式。
84%的病例可见膜 CD44v6 表达，81%的病例可见细胞质 CD44v6 表达。在 59%的膜 CD44v6 表达阳性的肿瘤中，浸润前缘的染色模式被确定为“前缘阳性”，而在 41%的肿瘤中为“前缘阴性”。与“前缘阳性”肿瘤相比，后者的模式与更窄的环周切缘（P=0.01）、浸润性生长模式（P<0.001）和无病生存时间更短有关（单因素生存分析，P=0.022）。
直肠癌浸润前缘缺乏膜 CD44v6 可作为识别复发风险增加患者的一种方法。
