Hampras Shalaka S, Nesline Mary, Wallace Paul K, Odunsi Kunle, Furlani Nicholas, Davis Warren, Moysich Kirsten B
Department of Cancer Prevention and Control, Roswell Park Cancer Institute, Buffalo, NY 14263, USA.
J Cancer Epidemiol. 2012;2012:191090. doi: 10.1155/2012/191090. Epub 2012 Aug 26.
Immunosuppressive regulatory T (Treg) cells play an important role in antitumor immunity, self-tolerance, transplantation tolerance, and attenuation of allergic response. Higher proportion of Treg cells has been observed in peripheral blood of cancer cases compared to controls. Little is known about potential epidemiological predictors of Treg cell levels in healthy individuals. We conducted a cross-sectional study including 75 healthy women, between 20 and 80 years of age, who participated in the Data Bank and BioRepository (DBBR) program at Roswell Park Cancer Institute (RPCI), Buffalo, NY, USA. Peripheral blood levels of CD4(+)CD25(+)FOXP3(+) Treg cells were measured using flow cytometric analysis. A range of risk factors was evaluated using Wilcoxon Rank-Sum test, Kruskal-Wallis test, and linear regression. Age, smoking, medications for treatment of osteoporosis, postmenopausal status, body mass index (BMI), and hormone replacement therapy (HRT) were found to be significant positive predictors of Treg cell levels in peripheral blood (P ≤ 0.05). Higher education, exercise, age at first birth, oral contraceptives, and use of Ibuprofen were found be significant (P < 0.05) negative predictors of Treg levels. Thus, various epidemiological risk factors might explain interindividual variation in immune response to pathological conditions, including cancer.
免疫抑制性调节性T（Treg）细胞在抗肿瘤免疫、自身耐受、移植耐受及减轻过敏反应中发挥着重要作用。与对照组相比，癌症患者外周血中Treg细胞比例更高。目前对于健康个体中Treg细胞水平的潜在流行病学预测因素知之甚少。我们开展了一项横断面研究，纳入了75名年龄在20至80岁之间、参与了美国纽约州布法罗布法罗罗斯威尔帕克癌症研究所（RPCI）数据库和生物样本库（DBBR）项目的健康女性。采用流式细胞术分析测定外周血中CD4(+)CD25(+)FOXP3(+) Treg细胞水平。使用Wilcoxon秩和检验、Kruskal-Wallis检验和线性回归评估一系列风险因素。结果发现，年龄、吸烟、骨质疏松治疗药物、绝经后状态、体重指数（BMI）和激素替代疗法（HRT）是外周血中Treg细胞水平的显著正预测因素（P≤0.05）。高等教育、运动、初产年龄、口服避孕药和布洛芬的使用是Treg水平的显著（P<0.05）负预测因素。因此，各种流行病学风险因素可能解释个体对包括癌症在内的病理状况免疫反应的个体差异。
