Cheng Jing-Lin, Wang Ai-Ling, Wan Jun
Department of Emergency, Second Affiliated Hospital of Anhui Medical University, Hefei 230601;
Exp Ther Med. 2012 Mar;3(3):509-512. doi: 10.3892/etm.2011.433. Epub 2011 Dec 23.
The aim of the present study was to explore the association between the M235T polymorphism of the angiotensinogen (AGT) gene and cytokines in patients with essential hypertension (EH). A total of 300 patients with EH and an age-matched control group of 150 individuals without EH, secondary hypertension, myocardial infarction and diabetes were enrolled in this study. Polymerase chain reaction combined with restriction fragment length polymorphism (PCR-RFLP) was used to detect variation in the target genotype, and enzyme-linked immunosorbant assay (ELISA) was used to detect the cytokine [interleukin (IL)-1, IL-6 and tumor necrosis factor-α (TNF-α)] concentrations. The AGT gene 235T allele and 235TT genotype frequencies in hypertensive patients were slightly higher than those in the controls. Furthermore, in the hypertensive subjects with the AGT gene 235T allele, the concentrations of IL-1 and TNF-α were significant higher than those in the controls. The results from our study suggest that the higher AGT gene TT genotype and 235T allele frequencies may be risk factors for hypertension. High frequencies of the AGT gene 235T allele and high cytokine concentrations (IL-1 and TNF-α) may promote the transcription and expression of AGT, particularly in hypertensive patients with the 235TT genotype.
本研究的目的是探讨原发性高血压（EH）患者血管紧张素原（AGT）基因M235T多态性与细胞因子之间的关联。本研究共纳入300例EH患者以及150例年龄匹配的对照个体，这些对照个体无EH、继发性高血压、心肌梗死和糖尿病。采用聚合酶链反应联合限制性片段长度多态性（PCR-RFLP）检测目标基因型的变异，并采用酶联免疫吸附测定（ELISA）检测细胞因子[白细胞介素（IL）-1、IL-6和肿瘤坏死因子-α（TNF-α）]浓度。高血压患者中AGT基因235T等位基因和235TT基因型频率略高于对照组。此外，在携带AGT基因235T等位基因的高血压受试者中，IL-1和TNF-α的浓度显著高于对照组。我们的研究结果表明，较高的AGT基因TT基因型和235T等位基因频率可能是高血压的危险因素。AGT基因235T等位基因的高频率和高细胞因子浓度（IL-1和TNF-α）可能促进AGT的转录和表达，尤其是在携带235TT基因型的高血压患者中。
