Chiang Yeunpo, Song Yongxi, Wang Zhenning, Liu Zhuangkai, Gao Peng, Liang Jiwang, Zhu Jinliang, Xing Chengzhong, Xu Huimian
Department of Surgical Oncology and General Surgery, First Hospital of China Medical University, Shenyang 110001, P.R. China.
Exp Ther Med. 2012 Mar;3(3):560-566. doi: 10.3892/etm.2011.436. Epub 2011 Dec 28.
microRNAs (miRNAs) are small, non-coding RNAs of endogenous origin. They have been increasingly shown to have aberrant expression in a number of tumor types. miR-192, -194 and -215 have not been comprehensively investigated using a large number of cases in colorectal cancer (CRC). We extracted total RNA from 107 CRC tissues and three CRC cell lines. Following polyadenylation and reverse transcription, the expression levels of miR-192, -194 and -215 were determined for evaluation of the association between expression levels and clinicopathological characteristics by a quantitative real-time polymerase chain reaction (real-time PCR) method. Finally, we studied the impact of miR-194 on cell proliferation in HCT-116 cells by MTT assay. miR-192, -194 and -215 were significantly downregulated in CRC tissues (all p<0.001, paired t-test) and cancer cell lines (all p<0.05) compared to non-tumor counterparts. Moreover, the expression levels of miR-192, -194 and -215 were demonstrated to be associated with increased tumor sizes (p=0.027, p=0.018, and p=0.027, respectively; Mann-Whitney U test). Also, there were marked correlations among these miRNAs in CRC tissues (all p<0.001, Pearson's regression analysis). Furthermore, we found that the overexpression of miR-194 could significantly inhibit cell proliferation in HTC-116 cells. miR-192, -194 and -215 may be important biological markers as tumor suppressors in the carcinogenesis of CRC.
微小RNA（miRNA）是内源性的小非编码RNA。越来越多的研究表明，它们在多种肿瘤类型中存在异常表达。在结直肠癌（CRC）中，尚未使用大量病例对miR-192、-194和-215进行全面研究。我们从107例CRC组织和3种CRC细胞系中提取了总RNA。经过聚腺苷酸化和逆转录后，采用定量实时聚合酶链反应（实时PCR）方法测定miR-192、-194和-215的表达水平，以评估表达水平与临床病理特征之间的关联。最后，我们通过MTT法研究了miR-194对HCT-116细胞增殖的影响。与非肿瘤对照相比，miR-192、-194和-215在CRC组织（所有p<0.001，配对t检验）和癌细胞系（所有p<0.05）中均显著下调。此外，miR-192、-194和-215的表达水平与肿瘤大小增加相关（分别为p=0.027、p=0.018和p=0.027；Mann-Whitney U检验）。而且，这些miRNA在CRC组织中存在显著相关性（所有p<0.001，Pearson回归分析）。此外，我们发现miR-194的过表达可显著抑制HTC-116细胞的增殖。miR-192、-194和-215可能作为肿瘤抑制因子，在CRC的致癌过程中是重要的生物学标志物。
