Salinas-Santander Mauricio, Díaz-García Daniel, Rojas-Martínez Augusto, Cantú-Salinas Cristina, Sánchez-Domínguez Celia, Reyes-López Miguel, Cerda-Flores Ricardo M, Ocampo-Candiani Jorge, Ortiz-López Rocío
Department of Biochemistry and Molecular Medicine, Faculty of Medicine, Universidad Autónoma de Nuevo León;
Exp Ther Med. 2012 May;3(5):893-897. doi: 10.3892/etm.2012.508. Epub 2012 Mar 9.
Vitiligo is a skin disease characterized by depigmentation. Its etiopathogenesis is unclear, but it has been associated with autoimmune processes. Gene polymorphisms in the tumor necrosis factor-α (TNF-α) have been associated with several imflammatory diseases. In particular, the -308G/A polymorphism in the gene promoter region has been reported to be associated with increased plasma levels of TNF-α and with an increased risk to develop autoimmune diseases. To date, this polymorphism has not been associated with vitiligo. To assess a possible association between the TNF-α -308G/A and vitiligo vulgaris (VV), 198 vitiligo patients and 395 control subjects were recruited for the study. A complete demographic and clinical profile of each case was registered to analyze the possible risk factors of vitiligo. Genomic DNA isolated from peri pheral blood was subjected to PCR-RFLP for genotyping of the TNF-α -308G/A polymorphism. Causal associations were determined by χ(2) test and their respective OR was assessed in a 2×2 contingency table. When population variables of type of vitiligo, gender, age of disease onset, and active disease status were considered, an association between active VV and the TNF-α GA genotype was found (P=0.0295, OR=2.0; 95% CI 1.01-3.93). All other variables were irrelevant to vitiligo. Our data suggest a possible association between the TNF-α -308 GA genotype and the active form of VV in a Mexican population.
白癜风是一种以色素脱失为特征的皮肤病。其发病机制尚不清楚，但与自身免疫过程有关。肿瘤坏死因子-α（TNF-α）基因多态性与多种炎症性疾病有关。特别是，该基因启动子区域的-308G/A多态性据报道与TNF-α血浆水平升高以及自身免疫性疾病发病风险增加有关。迄今为止，这种多态性尚未与白癜风相关联。为了评估TNF-α -308G/A与寻常型白癜风（VV）之间可能存在的关联，本研究招募了198例白癜风患者和395名对照受试者。记录了每个病例完整的人口统计学和临床资料，以分析白癜风可能的危险因素。从外周血中分离的基因组DNA进行PCR-RFLP，用于TNF-α -308G/A多态性的基因分型。通过χ²检验确定因果关联，并在2×2列联表中评估其各自的OR值。当考虑白癜风类型、性别、发病年龄和疾病活动状态等人群变量时，发现活动期VV与TNF-α GA基因型之间存在关联（P = 0.0295，OR = 2.0；95% CI 1.01 - 3.93）。所有其他变量与白癜风无关。我们的数据表明，在墨西哥人群中，TNF-α GA基因型与活动期VV之间可能存在关联。
