Rapoport I, Berger H, Elsner R, Rapoport S M
Acta Biol Med Ger. 1977;36(3-4):515-21.
A systematic study of the pH dependent changes in the range 6.6--7.4 of 2,3 bisphosphoglycerate (2,3-DPG) was performed in the presence and absence of glucose during transitional and steady states. The results indicate that 2,3-DPGase breaks down 2,3-DPG nealy independent of pH at a rate of 480 mu moles 2,3-DPG/1 cells.h. The 2,3-DPG mutase is practically completely inhibited below pH 6.9. The 2,3-DPG level in the presence of glucose reaches a pH dependent steady state after about 18 h. The share of the 2,3-DPG bypass in the steady state decreases from 24% at pH 7.4 to 12% at pH 7.0. The formation of pyruvate corresponds to the beadkdown of 2,3-DPG after consumption of an unknown reducing substance.
在有葡萄糖和无葡萄糖存在的情况下，分别在过渡态和稳态时，对2,3 - 二磷酸甘油酸（2,3 - DPG）在6.6 - 7.4范围内随pH值变化进行了系统研究。结果表明，2,3 - DPG酶以480微摩尔2,3 - DPG/1细胞·小时的速率分解2,3 - DPG，其分解速率几乎与pH值无关。在pH值低于6.9时，2,3 - DPG变位酶几乎完全被抑制。在有葡萄糖存在的情况下，2,3 - DPG水平在约18小时后达到pH值依赖的稳态。在稳态时，2,3 - DPG旁路的占比从pH 7.4时的24%降至pH 7.0时的12%。在消耗一种未知还原物质后，丙酮酸的形成与2,3 - DPG的分解相对应。
