Brand K, Quadflieg K H
Acta Biol Med Ger. 1977;36(3-4):507-13.
Metabolism of the substrates D-ribose, xylitol, D-Xylulose, D-fructose, D-glucose and mixtures of these compounds were studied in human erythrocytes. The metabolic rates obtained with the various substrates affected the intracellular levels of ATP and 2,3-bisphosphoglycerate. Small amounts of substrate utilization resulted in a decrease of the ATP and more pronounced of the 2,3-bisphosphoglycerate concentration while carbon utilization rates beyound 14 microgram atom C/ml packed cells/120 min yielded constant levels of ATP and 2,3-bisphosphoglycerate. From these results it can be concluded that a carbon utilization rate of 14 microgram atom C/ml cells/120 min is able to cover the ATP requirement of the red cells under steady state conditions. Based on the carbon utilization rates obtained with the various substrates and the rates of 2,3-bisphosphoglycerate decomposition an attempt is made to calculate the contribution of the 2,3-bisphosphoglycerate bypass to substrate metabolism. In case of xylitol as substrate the decrease in the 2,3-bisphosphoglycerate content provides the regeneration of NAD thus facilitating uptake and metabolism of xylitol.
在人红细胞中研究了底物D-核糖、木糖醇、D-木酮糖、D-果糖、D-葡萄糖以及这些化合物混合物的代谢。用各种底物获得的代谢率影响了细胞内ATP和2,3-二磷酸甘油酸的水平。少量底物利用导致ATP减少，2,3-二磷酸甘油酸浓度下降更明显，而碳利用率超过14微克原子C/毫升压积细胞/120分钟时，ATP和2,3-二磷酸甘油酸水平保持恒定。从这些结果可以得出结论，在稳态条件下，14微克原子C/毫升细胞/120分钟的碳利用率能够满足红细胞对ATP的需求。基于用各种底物获得的碳利用率和2,3-二磷酸甘油酸分解速率，尝试计算2,3-二磷酸甘油酸旁路对底物代谢的贡献。以木糖醇为底物时，2,3-二磷酸甘油酸含量的降低提供了NAD的再生，从而促进了木糖醇的摄取和代谢。
