Department of Chemical, Materials, and Biomolecular Engineering, School of Engineering, University of Connecticut, Storrs, Connecticut 06269, United States.
J Am Chem Soc. 2012 Sep 26;134(38):15716-9. doi: 10.1021/ja307717w. Epub 2012 Sep 14.
The ability to regulate cell-material interactions is important in various applications such as regenerative medicine and cell separation. This study successfully demonstrates that the binding states of cells on a hydrogel surface can be programmed by using hybridized aptamers and triggering complementary sequences (CSs). In the absence of the triggering CSs, the aptamers exhibit a stable, hybridized state in the hydrogel for cell-type-specific catch. In the presence of the triggering CSs, the aptamers are transformed into a new hybridized state that leads to the rapid dissociation of the aptamers from the hydrogel. As a result, the cells are released from the hydrogel. The entire procedure of cell catch and release during the transformation of the aptamers is biocompatible and does not involve any factor destructive to either the cells or the hydrogel. Thus, the programmable hydrogel is regenerable and can be applied to a new round of cell catch and release when needed.
调控细胞-材料相互作用的能力在再生医学和细胞分离等各种应用中非常重要。本研究成功地证明，通过使用杂交适体和触发互补序列（CSs），可以对水凝胶表面上细胞的结合状态进行编程。在不存在触发 CSs 的情况下，适体在水凝胶中表现出稳定的杂交状态，用于细胞类型特异性捕获。在存在触发 CSs 的情况下，适体转变为新的杂交状态，导致适体从水凝胶中迅速解离。结果，细胞从水凝胶中释放出来。适体转变过程中的细胞捕获和释放的整个过程是生物相容的，不会涉及任何对细胞或水凝胶具有破坏性的因素。因此，可编程水凝胶是可再生的，并且在需要时可以应用于新一轮的细胞捕获和释放。
