State Key Laboratory of Marine Resource Utilization in South China Sea, College of Materials and Chemical Engineering , Hainan University , Haikou 570228 , PR China.
ACS Appl Mater Interfaces. 2018 Mar 14;10(10):8546-8554. doi: 10.1021/acsami.8b00191. Epub 2018 Mar 2.
Various hydrogels have been used for protein delivery in the treatment of human diseases. Nevertheless, it is always difficult to control the capture and release of multiple proteins in different regions and periods. This research successfully proves that multiple proteins can be captured and released from the aptamer-patterned hydrogel films with an adjustable release rate at a prospective time and in specific regions utilizing the complementary DNA strand of aptamers via photoclick chemistry and DNA hybridization. The hydrogel film is successfully applied to complex matrixes such as human serum and has excellent cytocompatibility. Thus, the aptamer-patterned hydrogel film will be a good candidate for controlled delivery of multiple proteins.
多种水凝胶已被用于治疗人类疾病的蛋白质递送。然而，控制多种蛋白质在不同区域和时期的捕获和释放始终具有挑战性。本研究成功证明，通过光点击化学和 DNA 杂交，利用适体的互补 DNA 链，可以从适体图案化水凝胶膜中以可调节的释放速率在预期时间和特定区域捕获和释放多种蛋白质。水凝胶膜成功地应用于复杂基质，如人血清，具有优异的细胞相容性。因此，适体图案化水凝胶膜将是控制多种蛋白质递释的良好候选物。
