European Commission, Joint Research Center, Institute for Reference Materials and Measurements, Geel, Belgium.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2012;29(12):1959-71. doi: 10.1080/19440049.2012.720035. Epub 2012 Sep 13.
Recently, the use of substances that can suppress or reduce absorption, promote the excretion of mycotoxins or modify their mode of action in feed, so-called mycotoxin binders, has been officially allowed in the European Union as technological feed additives. The influence of the addition of mycotoxin binders to animal feed on the analytical performance of the official methods for the determination of mycotoxins was studied and the results are presented. Where possible standardised methods for analysis were applied. Samples of 20 commercial mycotoxin binders were collected from various companies. The following mycotoxins were included in the study: aflatoxin B₁, deoxynivalenol, zearalenone, ochratoxin A, fumonisins B₁ and B₂, T-2 and HT-2 toxins. A binder (or binders combined in a group) was mixed with feed material containing the mycotoxin, and the feed material was analysed. For data evaluation, the mean values were compared by Student's t-test (an independent two-sample t-test with unequal sample sizes and equal variance). The repeatability standard deviation of each method was used as an estimate of method variability. No significant differences (p = 0.05) in mycotoxin levels between binder-free material and the material containing different binders were found. Further, the possible effects of binder addition in combination with processing (pelletising) on the amount of aflatoxin B₁ determined in feed were studied. Three commercial mycotoxin binders containing hydrated sodium calcium aluminosilicate (HSCAS) as the main component were used in these experiments. Feed samples with and without mycotoxin binders were pelletised with and without steam treatment. After pelletising, materials were analysed for AFB₁. Only the combination pelletising and a mixture of binders added at a total level of 1.2% had a significant effect (41% reduction) on the amount of AFB₁ determined.
最近，欧盟正式允许将能够抑制或减少霉菌毒素吸收、促进霉菌毒素排泄或改变其在饲料中作用方式的物质，即霉菌毒素结合剂，作为一种技术饲料添加剂使用。本文研究了在动物饲料中添加霉菌毒素结合剂对官方霉菌毒素检测方法分析性能的影响，并给出了研究结果。尽可能采用了标准化的分析方法。从不同公司收集了 20 种市售霉菌毒素结合剂的样品。研究中包含了以下霉菌毒素：黄曲霉毒素 B₁、脱氧雪腐镰刀菌烯醇、玉米赤霉烯酮、赭曲霉毒素 A、伏马菌素 B₁ 和 B₂、T-2 和 HT-2 毒素。将结合剂（或结合剂组合在一起）与含有霉菌毒素的饲料材料混合，并对饲料材料进行分析。为了进行数据评估，通过 Student's t 检验（独立两样本 t 检验，样本大小不等，方差相等）比较平均值。每个方法的重复性标准差用作方法变异性的估计值。未发现无结合剂材料与含有不同结合剂的材料之间霉菌毒素水平存在显著差异（p = 0.05）。此外，还研究了结合剂添加与加工（制粒）相结合对饲料中黄曲霉毒素 B₁含量的可能影响。这些实验中使用了三种含有水合硅铝酸钠钙（HSCAS）作为主要成分的市售霉菌毒素结合剂。有和没有霉菌毒素结合剂的饲料样品在有和没有蒸汽处理的情况下进行制粒。制粒后，对 AFB₁进行了分析。只有制粒和以 1.2%的总水平添加混合物的结合剂组合对测定的 AFB₁量有显著影响（降低了 41%）。
