De Mil Thomas, Devreese Mathias, De Baere Siegrid, Van Ranst Eric, Eeckhout Mia, De Backer Patrick, Croubels Siska
Department of Pharmacology, Toxicology and Biochemistry, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
Department of Geology and Soil Science, Faculty of Science, Ghent University, Krijgslaan 281, S8, 9000 Ghent, Belgium.
Toxins (Basel). 2015 Jan 5;7(1):21-33. doi: 10.3390/toxins7010021.
The aim of this study was to characterize 27 feed additives marketed as mycotoxin binders and to screen them for their in vitro zearalenone (ZEN) adsorption. Firstly, 27 mycotoxin binders, commercially available in Belgium and The Netherlands, were selected and characterized. Characterization was comprised of X-ray diffraction (XRD) profiling of the mineral content and d-spacing, determination of the cation exchange capacity (CEC) and the exchangeable base cations, acidity, mineral fraction, relative humidity (RH) and swelling volume. Secondly, an in vitro screening experiment was performed to evaluate the adsorption of a single concentration of ZEN in a ZEN:binder ratio of 1:20,000. The free concentration of ZEN was measured after 4 h of incubation with each of the 27 mycotoxin binders at a pH of 2.5, 6.5 and 8.0. A significant correlation between the free concentration of ZEN and both the d-spacing and mineral fraction of the mycotoxin binders was seen at the three pH levels. A low free concentration of ZEN was demonstrated using binders containing mixed-layered smectites and binders containing humic acids.
本研究的目的是对27种作为霉菌毒素吸附剂销售的饲料添加剂进行特性分析，并筛选它们对玉米赤霉烯酮（ZEN）的体外吸附能力。首先，选取并表征了比利时和荷兰市场上可买到的27种霉菌毒素吸附剂。特性分析包括对矿物质含量和d间距进行X射线衍射（XRD）分析、测定阳离子交换容量（CEC）和可交换碱阳离子、酸度、矿物质组分、相对湿度（RH）以及膨胀体积。其次，进行了一项体外筛选实验，以评估在ZEN与吸附剂比例为1:20000的情况下，单一浓度的ZEN的吸附情况。在pH值为2.5、6.5和8.0的条件下，将27种霉菌毒素吸附剂中的每一种与ZEN孵育4小时后，测定ZEN的游离浓度。在这三个pH水平下，均观察到ZEN的游离浓度与霉菌毒素吸附剂的d间距和矿物质组分之间存在显著相关性。使用含有混合层蒙脱石的吸附剂和含有腐殖酸的吸附剂时，ZEN的游离浓度较低。
