蛋白质印迹法是用于副球孢子菌病和肺结核鉴别诊断的有效工具。
Western blotting is an efficient tool for differential diagnosis of paracoccidioidomycosis and pulmonary tuberculosis.
作者信息
Bertoni Thâmara Aline, Perenha-Viana Maysa Cláudia Zolin, Patussi Eliana Valéria, Cardoso Rosilene Fressatti, Svidzinski Terezinha Inez Estivalet
机构信息
Laboratory of Medical Mycology, Department of Clinical Analysis, Universidade Estadual de Maringá, Maringá, PR, Brazil.
出版信息
Clin Vaccine Immunol. 2012 Nov;19(11):1887-8. doi: 10.1128/CVI.00252-12. Epub 2012 Sep 12.
Abstract
Sputum and sera from 134 patients screened for tuberculosis (TB) were analyzed to investigate TB and paracoccidioidomycosis (PCM). Of these patients, 11 (8.2%) were confirmed to have TB, but six (4.5%) were positive only for PCM. All patients with PCM presented anti-43-kDa-component antibodies in Western blotting (WB) assays, while in the TB-positive patients these antibodies did not appear. This preliminary study suggests WB as a potential tool for differential laboratory diagnosis between TB and PCM.
摘要
对134名接受结核病（TB）筛查的患者的痰液和血清进行分析，以研究结核病和副球孢子菌病（PCM）。在这些患者中，11名（8.2%）被确诊患有结核病，但6名（4.5%）仅PCM呈阳性。所有PCM患者在蛋白质印迹（WB）检测中均呈现抗43-kDa成分抗体，而TB阳性患者中未出现这些抗体。这项初步研究表明WB可作为TB和PCM鉴别实验室诊断的潜在工具。
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