Universidade Federal do Espírito Santo, Avenida Marechal Campos, 1468, Maruípe, Vitória, ES, CEP 29043-900, Brazil.
Mol Biol Rep. 2012 Dec;39(12):10157-65. doi: 10.1007/s11033-012-1889-0. Epub 2012 Sep 13.
Human N-myc downstream-regulated gene 1 (NDRG1) is a metastasis suppressor gene with several potential functions, including cell differentiation, cell cycle regulation and response to hormones, nickel and stress. The purpose of this study was to investigate the immunoexpression of NDRG1 in oral and oropharyngeal squamous cell carcinomas searching for its role in the clinical course of these tumors. We investigated immunohistochemical expression of NDRG1 protein in 412 tissue microarray cores of tumor samples from 103 patients with oral and oropharyngeal squamous cell carcinomas and in 110 paraffin-embedded surgical margin sections. The results showed NDRG1 up-regulation in 101/103 (98.1 %) tumor samples, but no expression in any normal tissue sample. Western blot assays confirmed the immunohistochemical findings, suggesting that lower levels of NDRG1 are associated with a high mortality rate. NDRG1 overexpression was related to long-term specific survival (HR = 0.38; p = 0.009), whereas the presence of lymph-node metastasis showed the opposite association with survival (HR = 2.45; p = 0.013). Our findings reinforce the idea that NDRG1 plays a metastasis suppressor role in oral and oropharyngeal squamous cell carcinomas and may be a useful marker for these tumors.
人类 N- MYC 下游调节基因 1（NDRG1）是一种转移抑制基因，具有几种潜在的功能，包括细胞分化、细胞周期调控和对激素、镍和应激的反应。本研究旨在探讨 NDRG1 在口腔和口咽鳞状细胞癌中的免疫表达，以寻找其在这些肿瘤临床病程中的作用。我们研究了 103 例口腔和口咽鳞状细胞癌患者的 412 个组织微阵列核心肿瘤样本和 110 个石蜡包埋手术切缘切片中 NDRG1 蛋白的免疫组织化学表达。结果显示，103 例肿瘤样本中有 101 例（98.1%）出现 NDRG1 上调，但在任何正常组织样本中均无表达。Western blot 检测证实了免疫组织化学结果，表明 NDRG1 水平较低与高死亡率相关。NDRG1 过表达与长期特定生存率相关（HR = 0.38；p = 0.009），而淋巴结转移的存在与生存率呈相反的关联（HR = 2.45；p = 0.013）。我们的研究结果强化了 NDRG1 在口腔和口咽鳞状细胞癌中发挥转移抑制作用的观点，并且可能是这些肿瘤的有用标志物。
