3B's Research Group-Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909 Taipas, Guimarães, Portugal.
Biomed Mater. 2012 Oct;7(5):054104. doi: 10.1088/1748-6041/7/5/054104. Epub 2012 Sep 12.
Nanocomposite films based on a chitosan blend with bioactive glass nanoparticles (BG-NPs) with different formulations, namely SiO(2):CaO:P(2)O(5)(mol.%) = 55:40:5 and SiO(2):CaO:P(2)O(5):MgO(mol.%) = 64:26:5:5 were produced in order to develop systems with applicability in guided tissue regeneration. The zeta (ζ)-potential of the BG-NPs containing magnesium was found to be lower than the other formulation and the corresponding composite with chitosan was the most hydrophilic. The bioactive character of the biomaterials was also assessed in vitro by immersion of the materials in simulated body fluid, followed by scanning electron microscopy (SEM) and energy-dispersive x-ray spectroscopy evaluations. SaOs-2 osteoblastic-like cells were seeded on the different nanocomposites and their behavior was followed by SEM observations, cytotoxicity assessments, DNA quantification and alkaline phosphatase analysis. The introduction of the inorganic component in the chitosan matrix had a positive effect on the biological response of the membranes. The developed nanocomposite films are potential candidates for regenerating damaged bone tissue and could be useful in orthopedic and maxillo-facial applications.
基于具有不同配方的生物活性玻璃纳米粒子（BG-NPs）与壳聚糖共混的纳米复合膜被制备，用于开发在引导组织再生中具有应用适用性的系统。含有镁的 BG-NPs 的 ζ-电位被发现低于其他配方，并且相应的壳聚糖复合材料具有最高的亲水性。通过将材料浸入模拟体液中，然后通过扫描电子显微镜（SEM）和能量色散 X 射线光谱评估，体外评估了生物材料的生物活性特征。将 SaOs-2 成骨样细胞接种到不同的纳米复合材料上，并通过 SEM 观察、细胞毒性评估、DNA 定量和碱性磷酸酶分析来跟踪它们的行为。在壳聚糖基质中引入无机成分对膜的生物学反应有积极影响。所开发的纳米复合膜是再生受损骨组织的潜在候选物，可用于骨科和颌面应用。
