Sergi Rachele, Cannillo Valeria, Boccaccini Aldo R, Liverani Liliana
Dipartimento di Ingegneria Enzo Ferrari, Università degli Studi di Modena e Reggio Emilia, Via P. Vivarelli 10, 41125 Modena, Italy.
Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, Cauerstr. 6, 91058 Erlangen, Germany.
Materials (Basel). 2020 Dec 11;13(24):5651. doi: 10.3390/ma13245651.
Chitosan fibers blended with polyethylene oxide (CHIT_PEO) and crosslinked with genipin were fabricated by electrospinning technique. Subsequently, CHIT_PEO bioactive glass composite electrospun mats were fabricated with the aim to achieve flexible structures with adequate mechanical properties and improved biological performance respect to CHIT_PEO fibers, for potential applications in wound healing. Three different compositions of bioactive glasses (BG) were selected and investigated: 45S5 BG, a Sr and Mg containing bioactive glass (BGMS10) and a Zn-containing bioactive glass (BGMS_2Zn). Particulate BGs (particles size < 20 μm) were separately added to the starting CHIT_PEO solution before electrospinning. The two recently developed bioactive glasses (BGMS10 and BGMS_2Zn) showed very promising biological properties in terms of bioactivity and cellular viability; thus, such compositions were added for the first time to CHIT_PEO solution to fabricate composite electrospun mats. The incorporation of bioactive glass particles and their distribution into CHIT_PEO fibers were assessed by SEM and FTIR analyses. Furthermore, CHIT_PEO composite electrospun mats showed improved mechanical properties in terms of Young's Modulus compared to neat CHIT_PEO fibers; on the contrary, the values of tensile strain at break (%) were comparable. Biological performance in terms of cellular viability was investigated by means of WST-8 assay and CHIT_PEO composite electrospun mats showed cytocompatibility and the desired cellular viability.
采用静电纺丝技术制备了与聚环氧乙烷共混（CHIT_PEO）并经京尼平交联的壳聚糖纤维。随后，制备了CHIT_PEO生物活性玻璃复合静电纺丝垫，目的是获得具有足够机械性能的柔性结构，并相对于CHIT_PEO纤维提高其生物学性能，以用于伤口愈合的潜在应用。选择并研究了三种不同组成的生物活性玻璃（BG）：45S5 BG、含锶和镁的生物活性玻璃（BGMS10）以及含锌的生物活性玻璃（BGMS_2Zn）。在静电纺丝前，将颗粒状BG（粒径<20μm）分别添加到起始的CHIT_PEO溶液中。最近开发的两种生物活性玻璃（BGMS10和BGMS_2Zn）在生物活性和细胞活力方面显示出非常有前景的生物学特性；因此，首次将这些组成添加到CHIT_PEO溶液中以制备复合静电纺丝垫。通过扫描电子显微镜（SEM）和傅里叶变换红外光谱（FTIR）分析评估生物活性玻璃颗粒在CHIT_PEO纤维中的掺入情况及其分布。此外，与纯CHIT_PEO纤维相比，CHIT_PEO复合静电纺丝垫在杨氏模量方面表现出改善的机械性能；相反，断裂伸长率（%）的值相当。通过WST-8测定法研究了细胞活力方面的生物学性能，CHIT_PEO复合静电纺丝垫显示出细胞相容性和所需的细胞活力。
