3B's Research Group-Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, P-4806-909 Taipas, Guimarães, Portugal.
Nanotechnology. 2011 Dec 9;22(49):494014. doi: 10.1088/0957-4484/22/49/494014. Epub 2011 Nov 21.
Bioactive glass nanoparticles (BG-NPs), based on both ternary (SiO(2)-CaO-P(2)O(5)) and binary (SiO(2)-CaO) systems, were prepared via an optimized sol-gel method. The pH of preparation and the effect of heat treatment temperature were evaluated, as well as the effect of suppressing P in the bioactivity ability of the materials. The morphology and composition of the BG-NPs were studied using FTIR, XRD and SEM. The bioactive character of these materials was accessed in vitro by analyzing the ability for apatite formation onto the surface after being immersed in simulated body fluid (SBF). XRD, EDX and SEM were used to confirm the bioactivity of the materials. The BG-NP effect on cell metabolic activity was assessed by seeding L929 cells with their leachables, proving the non-cytotoxicity of the materials. Finally the most bioactive BG-NPs developed (ternary system prepared at pH 11.5 and treated at 700 °C) were successfully combined with chitosan in the production of biomimetic nanocomposite osteoconductive membranes that could have the potential to be used in guided tissue regeneration.
基于三元（SiO2-CaO-P2O5）和二元（SiO2-CaO）体系的生物活性玻璃纳米颗粒（BG-NPs），采用优化的溶胶-凝胶法制备。研究了制备的 pH 值和热处理温度的影响，以及抑制材料生物活性能力中 P 的作用。使用 FTIR、XRD 和 SEM 研究了 BG-NPs 的形貌和组成。通过分析在模拟体液（SBF）中浸泡后表面形成磷灰石的能力，在体外评估了这些材料的生物活性。使用 XRD、EDX 和 SEM 确认了材料的生物活性。通过用其浸提液接种 L929 细胞，评估 BG-NP 对细胞代谢活性的影响，证明了材料无细胞毒性。最后，成功地将开发的最具生物活性的 BG-NPs（在 pH 11.5 下制备的三元体系，在 700°C 下处理）与壳聚糖结合，生产仿生纳米复合骨传导膜，这些膜有可能用于引导组织再生。
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