Ishimoto Masaya, Sugimoto Naoko, Sekito Takayuki, Kawano-Kawada Miyuki, Kakinuma Yoshimi
Laboratory of Molecular Physiology and Genetics, Faculty of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime 790-8566, Japan.
Biosci Biotechnol Biochem. 2012;76(9):1802-4. doi: 10.1271/bbb.120372. Epub 2012 Sep 7.
Amino acid analysis of Saccharomyces cerevisiae cells indicated that neutral amino acids such as glycine and alanine were probably excluded from the vacuoles, and that vacuolar H(+)-ATPase (V-ATPase) was involved in the vacuolar compartmentalization of these amino acids. We found that vacuolar membrane vesicles export neutral amino acids in an ATP-dependent manner. This is important in identifying vacuolar transporters for neutral amino acids.
对酿酒酵母细胞的氨基酸分析表明，甘氨酸和丙氨酸等中性氨基酸可能被排除在液泡之外，并且液泡H(+)-ATP酶（V-ATP酶）参与了这些氨基酸的液泡区室化。我们发现液泡膜囊泡以ATP依赖的方式输出中性氨基酸。这对于鉴定中性氨基酸的液泡转运体很重要。
