Laboratory of Sustainable Environmental Biology, Graduate School of Agricultural Science, Tohoku University, 232–3 Yomogida, Naruko-onsen, Osaki, Miyagi 989–6711, Japan.
Microbes Environ. 2012;27(4):519-24. doi: 10.1264/jsme2.me12053. Epub 2012 Sep 5.
The distribution of ammonia-oxidizing archaea (AOA) and bacteria (AOB) in cattle, swine, and chicken manure compost was analyzed. PCR-denaturing gradient gel electrophoresis (DGGE) showed that a Candidatus Nitrososphaera gargensis-like sequence dominated in cattle manure compost, while few AOA were detected in other composts. In the case of AOB, Nitrosomonas-like sequences were detected with higher diversity in cattle and swine manure composts. The relative abundance of ammonia oxidizers by real-time PCR revealed that more AOB was present in compost except in one swine manure compost. Our results indicated that AOB rather than AOA are widely distributed in animal manure compost.
本研究分析了氨氧化古菌（AOA）和细菌（AOB）在牛粪、猪粪和鸡粪堆肥中的分布。PCR-变性梯度凝胶电泳（DGGE）显示，一种类似 Candidatus Nitrososphaera gargensis 的序列在牛粪堆肥中占主导地位，而其他堆肥中检测到的 AOA 较少。在 AOB 的情况下，检测到更多的硝化单胞菌样序列存在于牛粪和猪粪堆肥中，具有更高的多样性。实时 PCR 测定氨氧化菌的相对丰度表明，除了一个猪粪堆肥外，其他堆肥中都存在更多的 AOB。我们的结果表明，AOB 而不是 AOA 广泛分布于动物粪便堆肥中。
