Department of Neurology, Jiangyin Hospital of Traditional Chinese Medicine, Nanjing Traditional Chinese Medcine University, Jiangyin 214400, China.
Biol Pharm Bull. 2012;35(11):2004-9. doi: 10.1248/bpb.b12-00463. Epub 2012 Aug 22.
Stroke is the third leading cause of death in industrialized countries and the most important cause of acquired adult disability. Many evidences suggest that inflammation accounts for the progression of cerebral ischemic injury. Arctigenin, a phenylpropanoid dibenzylbutyrolactone lignin isolated from certain plants, has shown anti-inflammatory activity against diabetes and Alzheimer's disease. In this study, we tested whether arctigenin can protect middle cerebral artery occluded (MCAO) rats. Male Sprague-Dawley rats were pretreated with arctigenin or vehicle for 7 d before being subjected to transient occlusion of middle cerebral artery and reperfusion. Rats were evaluated at 24 h after MCAO for neurological deficit scoring. Furthermore, the mechanism of the anti-inflammatory effect of arctigenin was investigated with a focus on inflammatory cells, proinflammatory cytokines, and transcriptional factors. Arctigenin significantly reduced cerebral infarction and improved neurological outcome. Arctigenin suppressed the activation of microglia and decreased the expression of interleukin (IL)- 1β and tumor necrosis factor (TNF)-α. These results revealed that arctigenin has a promising therapeutic effect in ischemic stroke treatment through an anti-inflammatory mechanism.
中风是工业化国家的第三大致死原因，也是成年后天残疾的最重要原因。许多证据表明，炎症是导致脑缺血损伤进展的原因。牛蒡子苷，一种从某些植物中分离出的苯丙素二苄基丁内酯木脂素，已显示出对糖尿病和阿尔茨海默病的抗炎活性。在这项研究中，我们测试了牛蒡子苷是否可以保护大脑中动脉阻塞（MCAO）大鼠。雄性 Sprague-Dawley 大鼠在接受短暂大脑中动脉阻塞和再灌注前用牛蒡子苷或载体预处理 7 天。MCAO 后 24 小时对大鼠进行神经功能缺损评分评估。此外，还研究了牛蒡子苷的抗炎作用机制，重点关注炎症细胞、促炎细胞因子和转录因子。牛蒡子苷显著减少脑梗死面积，改善神经功能。牛蒡子苷抑制小胶质细胞的激活，降低白细胞介素（IL）-1β和肿瘤坏死因子（TNF）-α的表达。这些结果表明，牛蒡子苷通过抗炎机制在缺血性中风治疗中具有广阔的应用前景。
