Vasan N S, Lash J W
Calcif Tissue Res. 1975 Dec 18;19(2):99-107. doi: 10.1007/BF02563995.
Chondrocytes from 13-day-old embryonic chick sterna were cultured for 6 days in the presence of vitamin A (10 I.U./ml). Vitamin A treated chondrocytes became flattened and stellate within the first 24 hours of culture. After 6 days of culture, the treated cells contained 59% less DNA than the untreated controls. Sulfated glycosaminoglycan synthesis was inhibited 84%, and a greater percentage of GAG was secreted into the medium (90%) than in control cultures (78%). Vitamin A inhibited cell proliferation, and to varying degrees, RNA and protein synthesis, and these effects are dose dependent.
将13日龄胚胎鸡胸骨的软骨细胞在维生素A（10国际单位/毫升）存在的情况下培养6天。经维生素A处理的软骨细胞在培养的最初24小时内变扁平并呈星状。培养6天后，处理过的细胞所含DNA比未处理的对照细胞少59%。硫酸化糖胺聚糖的合成被抑制了84%，并且与对照培养物（78%）相比，有更大比例的糖胺聚糖分泌到培养基中（90%）。维生素A抑制细胞增殖，并且在不同程度上抑制RNA和蛋白质合成，这些作用呈剂量依赖性。
