Chi Fusheng, Fu Da, Zhang Xiaoping, Lv Zhongwei, Wang Zhesheng
Department of General Surgery, The First Hospital of China Medical University, Shenyang 110001, China.
Biosci Biotechnol Biochem. 2012;76(8):1471-6. doi: 10.1271/bbb.120132.
The hepatocyte growth factor receptor c-Met, a receptor tyrosine kinase, and Integrin α5β1, one of the main ECM receptors of hepatocytes have been reported to play important roles in tumor growth by activating mitogenic signaling pathways. In human gastric cardia adenocarcinoma, however, expression of the c-Met and Integrin α5β1 have not been reported. Here we examined the mRNA levels and protein expressions of these two genes and their relationship in human normal gastric cardia mucosa and primary carcinomas. Quantitative real-time PCR was used to analyze the mRNA expression of both c-Met and Integrin α5β1. The relationship between c-Met and Integrin α5β1 expression and the histologic characteristics of tumors were studied. Western blot analysis was performed to investigate the presence of c-Met and Integrin α5β1. The expression patterns of c-Met and Integrin α5β1 in 45 frozen slides of cardia adenocarcinoma were identified by immunohistochemistry. Our results indicate that the expression of c-Met and of Integrin α5β1 was significantly associated with tumor differentiation, TNM, and metastasis via the lymphogenic route. A significant positive correlation was also found between c-Met and Integrin α5β1 mRNA expression, suggesting that expression of c-Met and Integrin α5β1 has mechanical significance in the early stages of human gastric cardia adenocarcinoma.
肝细胞生长因子受体c-Met是一种受体酪氨酸激酶，而整合素α5β1是肝细胞主要的细胞外基质（ECM）受体之一，据报道它们通过激活促有丝分裂信号通路在肿瘤生长中发挥重要作用。然而，在人类胃贲门腺癌中，尚未见c-Met和整合素α5β1表达情况的报道。在此，我们检测了这两个基因在人类正常胃贲门黏膜和原发性癌中的mRNA水平、蛋白表达及其相互关系。采用定量实时PCR分析c-Met和整合素α5β1的mRNA表达。研究了c-Met和整合素α5β1表达与肿瘤组织学特征之间的关系。进行蛋白质印迹分析以检测c-Met和整合素α5β1的存在情况。通过免疫组织化学鉴定45张胃贲门腺癌冰冻切片中c-Met和整合素α5β1的表达模式。我们的结果表明，c-Met和整合素α5β1的表达与肿瘤分化、TNM分期以及淋巴源性转移显著相关。c-Met和整合素α5β1的mRNA表达之间也存在显著正相关，提示c-Met和整合素α5β1的表达在人类胃贲门腺癌早期具有重要意义。
