Department of Small Animal Disease with Clinic, Warsaw University of Life Sciences, Nowoursynowska 159c, 02-776 Warsaw, Poland.
Arch Microbiol. 2013 Jan;195(1):37-41. doi: 10.1007/s00203-012-0844-8. Epub 2012 Sep 14.
Staphylococcus aureus subsp. anaerobius is the etiological agent of the Morel's disease in sheep and goats. The disease presents with subcutaneous abscesses, located mainly in the superficial lymph nodes. Forty-one isolates of S. aureus subsp. anaerobius were collected from two outbreaks of the Morel's disease in Poland in years 2006-2008. Analysis of DNA SmaI digests by PFGE showed that 35 of 41 isolates belonged to the same PFGE type, identical to the type strain of S. aureus subsp. anaerobius ATCC 35844, confirming high level of clonality of the species. The DNA patterns of the remaining identical 6 isolates, different from the reference strain only by two bands, were found closely related. Genotyping performed with AFLP technique revealed two clonal groups including 16 and 25 isolates, respectively. The study indicated that AFLP technique might be a better discriminatory tool for genetic analysis of S. aureus subsp. anaerobius isolates, when compared to PFGE.
厌氧金黄色葡萄球菌亚种是绵羊和山羊梅莱尔氏病的病原体。该病以皮下脓肿为特征，主要位于浅表淋巴结。2006 年至 2008 年期间，在波兰发生的两次梅莱尔氏病疫情中，从 41 株厌氧金黄色葡萄球菌亚种中采集了 41 株。PFGE 对 SmaI 消化 DNA 的分析表明，41 株分离株中的 35 株属于同一 PFGE 型，与厌氧金黄色葡萄球菌亚种的参考菌株 ATCC 35844 相同，证实了该种的高克隆性。其余 6 株分离株的 DNA 模式与参考株仅相差两条带，发现它们密切相关。AFLP 技术进行的基因分型显示，分别有 16 株和 25 株分离株属于两个克隆群。研究表明，与 PFGE 相比，AFLP 技术可能是厌氧金黄色葡萄球菌亚种分离株遗传分析的更好区分工具。
