从波兰山羊中分离出的金黄色葡萄球菌厌氧亚种的多位点序列分型（MLST）和随机扩增多态性DNA（RAPD）分子分析
MLST and RAPD molecular analysis of Staphylococcus aureus subsp. anaerobius isolated from goats in Poland.
作者信息
Szaluś-Jordanow Olga, Krysztopa-Grzybowska Katarzyna, Czopowicz Michał, Moroz Agata, Mickiewicz Marcin, Lutyńska Anna, Kaba Jarosław, Nalbert Tomasz, Frymus Tadeusz
机构信息
Division of Infectious Diseases, Department of Small Animal Diseases with Clinic, Warsaw University of Life Sciences-SGGW, Nowoursynowska 159c Street, 02-776, Warsaw, Poland.
Department of Sera and Vaccine Evaluation, National Institute of Public Health-National Institute of Hygiene (NIPH-NIH), 24 Chocimska Street, 00-791, Warsaw, Poland.
出版信息
Arch Microbiol. 2018 Nov;200(9):1407-1410. doi: 10.1007/s00203-018-1568-1. Epub 2018 Sep 4.
Staphylococcus aureus subsp. anaerobius is an etiological agent of Morel's disease in small ruminants. The infection results in superficial abscesses located near lymph nodes. In the study, molecular analysis based on multilocus sequence typing (MLST) of seven housekeeping genes (arcC, aroE, glp, gmk, pta, tpi, yqiL) and random amplified polymorphic DNA (RAPD) was carried out on 19 S. aureus subsp. anaerobius strains isolated from two different goat herds from Poland. All of the 19 S. aureus subsp. anaerobius strains were found to belong to single MLST and RAPD types which support the high clonality level of this agent. However, the results obtained show clearly that the S. aureus subsp. anaerobius clone found in goats in Poland is different from those previously described. However, it is identical to the ATCC 38844 strain isolated from sheep in Spain, which has not been so far genotyped using MLST.
金黄色葡萄球菌厌氧亚种是小反刍动物莫雷尔病的病原体。感染会导致淋巴结附近出现浅表脓肿。在该研究中，对从波兰两个不同山羊群中分离出的19株金黄色葡萄球菌厌氧亚种菌株，基于七个管家基因（arcC、aroE、glp、gmk、pta、tpi、yqiL）的多位点序列分型（MLST）和随机扩增多态性DNA（RAPD）进行了分子分析。发现所有19株金黄色葡萄球菌厌氧亚种菌株都属于单一的MLST和RAPD类型，这支持了该病原体的高克隆性水平。然而，所获得的结果清楚地表明，在波兰山羊中发现的金黄色葡萄球菌厌氧亚种克隆与先前描述的不同。不过，它与从西班牙绵羊中分离出的ATCC 38844菌株相同，该菌株迄今为止尚未使用MLST进行基因分型。
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