利用高特异性单克隆抗体研究新型转录因子HMBOX1在人类癌症中的表达谱。
Expression profile of HMBOX1, a novel transcription factor, in human cancers using highly specific monoclonal antibodies.
作者信息
Dai Jun, Zhang Cai, Tian Zhigang, Zhang Jian
机构信息
Institute of Immunopharmacology and Immunotherapy, School of Pharmaceutical Sciences, Shandong University, Jinan, Shandong 250012, P.R. China.
出版信息
Exp Ther Med. 2011 May;2(3):487-490. doi: 10.3892/etm.2011.240. Epub 2011 Mar 21.
Homeobox containing 1 (HMBOX1) is a novel transcription factor. However, the expression of HMBOX1 and its functions in human cancer tissues and cell lines have not been fully defined. We generated two specific monoclonal antibodies, 2A5F4 and 4A4F2, against human HMBOX1. In the present study, these two anti-HMBOX1 antibodies were used to investigate the protein expression profile of HMBOX1 in various human cancer tissues and cell lines. The results showed that HMBOX1 in kidney tissue was mainly expressed in the renal tubule; the expression level of HMBOX1 was much higher in clear-cell carcinoma of the kidney originating from the renal tubule. Additionally, high levels of HMBOX1 protein were detected not only in pancreatic cancer tissue but also in the adjacent normal tissue. Notably, the expression level of HMBOX1 in liver cancer was dramatically decreased compared with that in the adjacent normal tissue. Abnormal expression of HMBOX1 in different types of carcinoma tissues suggests that HMBOX1 may be involved in the pathobiology of tumors.
含同源盒1（HMBOX1）是一种新型转录因子。然而，HMBOX1在人类癌组织和细胞系中的表达及其功能尚未完全明确。我们制备了两种针对人HMBOX1的特异性单克隆抗体，2A5F4和4A4F2。在本研究中，这两种抗HMBOX1抗体用于研究HMBOX1在各种人类癌组织和细胞系中的蛋白质表达谱。结果显示，肾组织中的HMBOX1主要表达于肾小管；起源于肾小管的肾透明细胞癌中HMBOX1的表达水平高得多。此外，不仅在胰腺癌组织中检测到高水平的HMBOX1蛋白，在相邻正常组织中也检测到。值得注意的是，与相邻正常组织相比，肝癌中HMBOX1的表达水平显著降低。HMBOX1在不同类型癌组织中的异常表达表明，HMBOX1可能参与肿瘤的病理生物学过程。
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