Ojeda F, Guarda M I, Maldonado C, Folch H
Department of Physics, Universidad Austral de Chile, Valdivia.
Cell Immunol. 1990 Feb;125(2):535-9. doi: 10.1016/0008-8749(90)90106-2.
The involvement of protein kinase-C in thymocytes death induced by hydrocortisone was studied. Thymus cells were incubated 6 hr or in the presence of hydrocortisone, labeled with Acridine orange, and the DNA content of each nuclei was estimated by cytofluorimetry. The results indicate that hydrocortisone-induced DNA fragmentation can be prevented by adding the protein kinase-C inhibitor H-7 to the cell suspension. Incubation of the H-A 1004, an inhibitor of c-AMP-dependent protein kinase, with low effect on on protein kinase-C, did not interfere with the cortisone-mediated DNA fragmentation. Therefore, it can be concluded that protein kinase-C plays an important role in the process of lympholysis mediated by corticoids.
研究了蛋白激酶-C在氢化可的松诱导胸腺细胞死亡中的作用。胸腺细胞在氢化可的松存在的情况下孵育6小时，用吖啶橙标记，然后通过细胞荧光测定法评估每个细胞核的DNA含量。结果表明，向细胞悬液中添加蛋白激酶-C抑制剂H-7可防止氢化可的松诱导的DNA片段化。H-A 1004是一种对c-AMP依赖性蛋白激酶有抑制作用但对蛋白激酶-C影响较小的物质，其孵育对可的松介导的DNA片段化没有干扰。因此，可以得出结论，蛋白激酶-C在皮质激素介导的淋巴细胞溶解过程中起重要作用。
