Love J T, Padula S J, Lingenheld E G, Amin J K, Sgroi D C, Wong R L, Sha'fi R I, Clark R B
Department of Medicine, University of Connecticut School of Medicine, Farmington 06032.
Biochem Biophys Res Commun. 1989 Jul 14;162(1):138-43. doi: 10.1016/0006-291x(89)91973-6.
Culturing murine T cell tumor lines in the presence of the protein kinase inhibitor H-7 for 4 days led to their dependence on H-7 for maximal constitutive proliferation. Withdrawal of H-7 from H-7-conditioned cells led to inhibition of proliferation and cell death. The mechanism underlying this H-7 dependence does not appear to be related to clonal selection or to effects on protein kinase C or the cyclic nucleotide-dependent kinases. This suggests that all the effects of the widely used H-7 may not be completely understood, and that H-7 may be useful in the dissection of the complex patterns of growth regulation in T cell malignancies.
