Politeo Olivera, Skocibusic Mirjana, Carev Ivana, Burcul Franko, Jerkovic Igor, Sarolic Mladenka, Milos Mladen
Department of Biochemistry, Faculty of Chemistry and Technology, University ofSplit, Teslina 10/V, 21000 Split, Croatia.
Nat Prod Commun. 2012 Aug;7(8):1087-90.
The volatile oils obtained from the leaves and flowers of the Croatian endemic plant Centaurea ragusina L. were evaluated for antimicrobial activity. The chemical compositions, determined by GC and GC-MS, were complex, with sesquiterpenes the most abundant class of compound. Nonterpene hydrocarbons were also identified in high quantities, while acids and ketones were found in high quantities only in the leaf volatile oil. Aldehydes, alcohols, esters and other compounds were identified in lower quantities. Antimicrobial effects of the volatile samples were investigated by using the disc diffusion and microdilution assays. They were found to inhibit a wide range of bacteria and fungi, causing in vitro growth inhibition at a concentration of 250 microg/mL. The volatile oils displayed great antibacterial potential with MIC values ranging from 16 to 128 microg/mL against the Gram-positive bacteria Bacillus cereus, Clostridium perfringens, Enterococcus faecalis and Staphylococcus aureus, and lesser activity against the Gram-negative bacteria Cronobacter sakazakii, Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa, as well as fungal strains (MIC = 32-128 microg/mL).
对从克罗地亚本土植物拉古西纳矢车菊（Centaurea ragusina L.）的叶子和花朵中提取的挥发油进行了抗菌活性评估。通过气相色谱（GC）和气相色谱 - 质谱联用（GC - MS）测定的化学成分复杂，倍半萜是最丰富的化合物类别。还大量鉴定出非萜类碳氢化合物，而酸和酮仅在叶挥发油中大量存在。醛、醇、酯和其他化合物的含量较低。通过纸片扩散法和微量稀释法研究了挥发油样品的抗菌效果。发现它们能抑制多种细菌和真菌，在浓度为250微克/毫升时导致体外生长抑制。挥发油对革兰氏阳性菌蜡样芽孢杆菌、产气荚膜梭菌、粪肠球菌和金黄色葡萄球菌显示出巨大的抗菌潜力，最低抑菌浓度（MIC）值范围为16至128微克/毫升，对革兰氏阴性菌阪崎肠杆菌、大肠杆菌、肺炎克雷伯菌和铜绿假单胞菌以及真菌菌株（MIC = 32 - 128微克/毫升）的活性较低。
