Grendel T, Sokolský J, Vaščáková A, Hudák V, Chovanec M, Sabol F, André S, Kaltner H, Gabius H-J, Frankovičová M, Lenčeš P, Betka J, Smetana K, Gál P
1st Department of Anesthesiology and Intensive Medicine, Pavol Jozef Šafárik University and Louis Pasteur University Hospital, Košice, Slovak Republic.
Folia Biol (Praha). 2012;58(4):135-43.
Tracheotomy may be associated with numerous acute and chronic complications including extensive formation of granulation tissue. The emerging functional versatility of the adhesion/growth-regulatory galectins prompted us to perform a histochemical study of wound healing using rat trachea as model. By using non-cross-reactive antibodies and the labelled tissue lectins we addressed the issue of the presence and regulation of galectin reactivity during trachea wound healing. Beside localization of high-molecular-weight keratin, wide-spectrum cytokeratin, keratins 10 and 14, α-smooth muscle actin, vimentin, fibronectin, and Sox-2, galectins -1, -2, and -3 and their reactivity profiles were measured in frozen sections of wounded and control trachea specimens 7, 14, and 28 days after trauma. A clear trend for decreased galectin-1 presence and increased reactivity for galectin-1 was revealed from day 7 to day 28. Sox-2-positive cells were present after seven days and found in the wound bed. Interestingly, several similarities were observed in comparison to skin wound healing including regulation of galectin-1 parameters.
气管切开术可能会引发多种急慢性并发症，包括肉芽组织的大量形成。黏附/生长调节半乳糖凝集素新出现的功能多样性促使我们以大鼠气管为模型进行伤口愈合的组织化学研究。通过使用非交叉反应抗体和标记的组织凝集素，我们探讨了气管伤口愈合过程中半乳糖凝集素反应性的存在和调节问题。除了定位高分子量角蛋白、广谱细胞角蛋白、角蛋白10和14、α平滑肌肌动蛋白、波形蛋白、纤连蛋白和Sox-2外，还在创伤后7天、14天和28天的受伤气管标本和对照气管标本的冰冻切片中测量了半乳糖凝集素-1、-2和-3及其反应谱。从第7天到第28天，半乳糖凝集素-1的存在明显减少，而其反应性增加。创伤7天后出现Sox-2阳性细胞，并在伤口床中发现。有趣的是，与皮肤伤口愈合相比，观察到了一些相似之处，包括半乳糖凝集素-1参数的调节。
