Department of Control and Computer Engineering, Politecnico di Torino, Italy.
Comput Biol Med. 2012 Oct;42(10):1012-25. doi: 10.1016/j.compbiomed.2012.08.004. Epub 2012 Sep 13.
Although immunohistochemistry (IHC) is a popular imaging technique, the quantitative analysis of IHC images via computer-aided methods is an emerging field that is gaining more and more importance thanks to the new developments in digital high-throughput scanners. In this paper, we discuss the main steps of IHC and review the techniques for computer-aided chromogenic IHC analysis, including methods to determine the location of interest of the antigens and quantify their activations. Moreover, we discuss the issues arising from the standardization of the immunostaining process, that are generally overlooked by the current literature, and finally provide requirements for reliable computer-aided IHC quantification.
尽管免疫组织化学（IHC）是一种流行的成像技术，但通过计算机辅助方法对 IHC 图像进行定量分析是一个新兴领域，由于数字高通量扫描仪的新发展，它变得越来越重要。在本文中，我们讨论了 IHC 的主要步骤，并回顾了计算机辅助显色 IHC 分析的技术，包括确定抗原感兴趣位置并量化其激活的方法。此外，我们还讨论了免疫染色过程标准化所带来的问题，这些问题通常被当前文献所忽视，最后为可靠的计算机辅助 IHC 定量提供了要求。
