School of Pharmacy, University of Camerino, Italy.
Food Chem. 2012 Dec 15;135(4):2852-62. doi: 10.1016/j.foodchem.2012.07.027. Epub 2012 Jul 16.
Smyrnium olusatrum, well known as wild celery or Alexanders, is a biennial herb belonging to the Apiaceae and used for many centuries as an edible vegetable because of the aromatic flavour of its parts. Its use on the table has decreased since the Middle Ages when it was replaced by common celery (Apium graveolens). In the present work the composition of the essential oils obtained by hydrodistillation of various plant parts was investigated by gas chromatography (GC-FID and GC-MS), while quantitation of a heat-sensitive compound, isofuranodiene, known for its anticancer activity, and of its Cope rearrangement product curzerene, was achieved by HPLC-DAD. All essential oils were composed mainly of furanosesquiterpenoids (54.1-76.2%) with isofuranodiene (19.5-45.8%) as the main constituent. Results showed that GC analysis provides underestimation (up to five times) of isofuranodiene levels in essential oils and that curzerene could be considered an artifact since it is produced during heating of plant material, occurring during hydrodistillation.
水芹，俗称野芹菜或洋芫荽，是伞形科的二年生草本植物，因其部分具有芳香气味，几个世纪以来一直被用作食用蔬菜。从中世纪以来，它的使用量有所减少，当时它被普通芹菜（Apium graveolens）所取代。在本研究中，通过气相色谱法（GC-FID 和 GC-MS）研究了水芹不同部位水蒸气蒸馏所得到的精油的组成，同时通过高效液相色谱法-二极管阵列检测法（HPLC-DAD）对热敏感化合物异呋喃并二烯（具有抗癌活性）及其 Cope 重排产物枯茗烯进行了定量分析。所有精油主要由呋喃倍半萜（54.1-76.2%）组成，异呋喃并二烯（19.5-45.8%）是主要成分。结果表明，GC 分析对精油中异呋喃并二烯的含量存在低估（高达五倍），并且枯茗烯可能被认为是一种人工产物，因为它是在植物材料加热过程中产生的，而这种加热过程发生在水蒸气蒸馏过程中。
