Quassinti Luana, Maggi Filippo, Barboni Luciano, Ricciutelli Massimo, Cortese Manuela, Papa Fabrizio, Garulli Chiara, Kalogris Cristina, Vittori Sauro, Bramucci Massimo
School of Pharmacy, University of Camerino, Italy.
School of Pharmacy, University of Camerino, Italy.
Fitoterapia. 2014 Sep;97:133-41. doi: 10.1016/j.fitote.2014.06.004. Epub 2014 Jun 9.
Smyrnium olusatrum (Apiaceae), well known as wild celery, is a biennal celery-scented plant used for many centuries as a vegetable, then abandoned after the introduction of celery. In the present work, the essential oil obtained from inflorescences and the amounts of its main constituents isofuranodiene, curzerene and germacrone were analyzed by GC as well as by HPLC because of their degradation (Cope rearrangement) occurring at high temperatures. The oil and the main constituents were assayed for cytotoxic activity on the human colon cancer cell line (HCT116) by MTT assay. Flower oil and isofuranodiene showed noteworthy activity on tumor cells with IC50 of 10.71 and 15.06 μg/ml, respectively. Analysis of the cytotoxic activity showed that wild celery oil and isofuranodiene are able to induce apoptosis in colon cancer cells in a time and concentration-dependent manner suggesting a potential role as models for the development of chemopreventive agents.
刺芹（伞形科），俗称野芹菜，是一种两年生的具有芹菜香味的植物，数百年来一直被用作蔬菜，在芹菜引入后就被弃用了。在本研究中，由于其主要成分异呋喃二烯、莪术烯和吉马酮在高温下会发生降解（科普重排），因此采用气相色谱法（GC）和高效液相色谱法（HPLC）对从花序中提取的精油及其主要成分的含量进行了分析。通过MTT法测定了该精油及其主要成分对人结肠癌细胞系（HCT116）的细胞毒性活性。花油和异呋喃二烯对肿瘤细胞显示出显著活性，IC50分别为10.71和15.06μg/ml。细胞毒性活性分析表明，野芹菜油和异呋喃二烯能够以时间和浓度依赖的方式诱导结肠癌细胞凋亡，提示其作为化学预防剂开发模型具有潜在作用。
