Department of Veterinary Parasitology, Babol-Branch, Islamic Azad University, Babol 19585/466, Iran.
Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol 4618649767, Iran.
Molecules. 2020 Aug 7;25(16):3593. doi: 10.3390/molecules25163593.
Cystic echinococcosis (CE) remains an important challenge both in humans and animals. There is no safe and suitable remedy for CE, so the discovery of new compounds with promising scolicidal effects, particularly from herbal sources, is of great importance for therapeutic uses in the treatment and prevention of CE reappearance. Sesquiterpenes are C15 organic compounds made up of three isoprene units and mostly occurring as fragrant components of essential oils. They are of economic importance for the cosmetic and pharmaceutical industry, and recently attracted the attention of the scientific community for their remarkable parasiticidal properties. In the present study, we have focused on three known sesquiterpenes, isofuranodiene (IFD), α-bisabolol (BSB), and farnesol (FOH), as important phytoconstituents of the essential oils of wild celery (), chamomile (), and acacia farnese (), respectively. Protoscoleces were recovered from fertile hydatid cysts and were exposed to different concentrations of the three tested compounds for different exposure times. The viability of protoscoleces was confirmed by 0.1% eosin staining. Results of scolicidal activity evaluations showed that IFD possessed the best effect against protoscoleces (LC and LC values of 8.87 and 25.48 µg/mL, respectively), followed by BSB (LC of 103.2 µg/mL) and FOH (LC of 113.68 µg/mL). The overall toxicity of IFD differed significantly from those of FOH and BSB, while there was no significant difference in toxicity between the latter compounds ( > 0.05). The present study showed that IFD seems to be a promising scolicidal agent and can be further tested to become a candidate for CE treatment.
棘球蚴病（CE）仍然是人类和动物面临的重要挑战。目前尚无安全且适用的 CE 治疗方法，因此，从草药来源中发现具有潜在杀棘球蚴作用的新化合物，对于 CE 复发的治疗和预防具有重要意义。倍半萜是由三个异戊二烯单元组成的 C15 有机化合物，主要作为精油的芳香成分存在。它们对化妆品和制药行业具有重要的经济意义，最近因其显著的寄生虫杀灭特性而引起科学界的关注。在本研究中，我们重点研究了三种已知的倍半萜，异呋哺烯（IFD）、α- 姜黄烯（BSB）和法呢醇（FOH），它们分别是野生芹菜（）、 洋甘菊（）和金合欢（）精油的重要植物成分。原头蚴是从有活力的包虫囊中回收的，并暴露于不同浓度的三种测试化合物中不同的暴露时间。原头蚴的活力通过 0.1%伊红染色得到确认。杀棘球蚴活性评价结果表明，IFD 对原头蚴的效果最好（LC 和 LC 值分别为 8.87 和 25.48 µg/mL），其次是 BSB（LC 为 103.2 µg/mL）和 FOH（LC 为 113.68 µg/mL）。IFD 的总体毒性与 FOH 和 BSB 有显著差异，而这两种化合物之间的毒性没有显著差异（> 0.05）。本研究表明，IFD 似乎是一种很有前途的杀棘球蚴剂，可以进一步测试成为 CE 治疗的候选药物。
