Villablanca J R, Shook B L, Hovda D A, Sutton R L
Department of Psychiatry, UCLA School of Medicine.
Dev Neurosci. 1990;12(1):1-10. doi: 10.1159/000111830.
Tissue from fetal frontal cortex survived after transplantation onto the surface of the left thalamus in 2 kittens and 2 adult cats which 7 days previously had sustained a left cerebral hemispherectomy. There were nerve fiber connections with host tissue (WGA-HRP, Loyez myelin stain) only in the neonatal animals. The grafts contained surviving neurons in all but in one adult cat which survived 301 days. The grafts had little effects on the retrograde ventral thalamic degeneration typically seen following hemispherectomy. However, the dorsal lateral geniculate nucleus adjacent to the transplants showed reduced neuronal loss and gliosis compared to controls. Magnetic resonance imaging was successfully used to visualize the grafts in vivo and suggested a decrease in size as well as changes in composition for a graft systematically followed for 120 days posttransplantation. Cytochrome oxidase histochemistry indicated sustained metabolic activity in transplants containing surviving neurons. This study introduces the cat as a useful model for brain tissue transplantation in a classical, myelinated sensorimotor system.
在2只幼猫和2只成年猫中，将胎儿额叶皮质组织移植到7天前接受了左大脑半球切除术的左丘脑表面后存活了下来。只有新生动物的移植组织与宿主组织存在神经纤维连接（WGA-HRP、Loyez髓鞘染色）。除了一只存活301天的成年猫外，所有移植物中均含有存活的神经元。移植物对半球切除术后典型出现的逆行性丘脑腹侧变性几乎没有影响。然而，与对照组相比，移植部位附近的背外侧膝状体核神经元丢失和胶质增生减少。磁共振成像成功用于在体内观察移植物，并且表明在移植后系统观察120天的移植物大小减小以及成分发生变化。细胞色素氧化酶组织化学表明，含有存活神经元的移植物具有持续的代谢活性。本研究将猫引入作为经典的、有髓鞘的感觉运动系统中脑组织移植的有用模型。
