Pasquini R, Monarca S, Scassellati Sforzolini G, Savino A, Fatigoni C, Puccetti P
Department of Hygiene, University of Perugia, Italy.
Environ Mol Mutagen. 1990;15(1):56-66. doi: 10.1002/em.2850150108.
A petroleum pitch sample collected in a carbon electrode factory was studied using a series of in vivo assays for genotoxicity and enzymatic induction capability. Rats were treated with the petroleum derivative in three doses: 100, 50, and 10 mg/kg body weight. The treatment produced a rapid excretion of mutagenic substances in the urines of the first 24 hr only in rats treated with high doses (100 and 50 mg/kg). No faecal mutagenic activity was observed. Analyses of urinary thioethers showed that urinary metabolites derived from the compounds present in the pitch-sample at the lowest dose-administered (10 mg/kg) were eliminated primarily as cysteine conjugates. The pitch sample was found to be a good inducer of pulmonary and hepatic aryl hydrocarbon hydroxylase, especially after a 50 mg/kg dose. Urinary D-glucaric acid content was always statistically increased in treated animals compared with controls, confirming the enzymatic induction activity. Hepatic glutathione-S-transferase activity increased following treatment with 50 and 10 mg/kg doses.
采用一系列体内遗传毒性检测和酶诱导能力检测方法，对在一家碳电极厂采集的石油沥青样品进行了研究。用三种剂量的石油衍生物对大鼠进行处理：100、50和10毫克/千克体重。仅在接受高剂量（100和50毫克/千克）处理的大鼠中，处理后的头24小时内尿液中诱变物质迅速排出。未观察到粪便诱变活性。尿硫醚分析表明，最低给药剂量（10毫克/千克）的沥青样品中所含化合物的尿代谢产物主要以半胱氨酸结合物的形式排出。发现该沥青样品是肺和肝芳烃羟化酶的良好诱导剂，尤其是在50毫克/千克剂量之后。与对照组相比，处理动物的尿D-葡糖醛酸含量在统计学上总是增加，证实了酶诱导活性。用50和10毫克/千克剂量处理后，肝谷胱甘肽-S-转移酶活性增加。
