Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, Iran.
Gene. 2012 Dec 1;510(2):113-7. doi: 10.1016/j.gene.2012.08.048. Epub 2012 Sep 9.
Metabolic syndrome (MeS) is associated with increased risk for type 2 diabetes mellitus (T2DM) and cardiovascular disease. There is some evidence indicating that adipokines play a role in the development of MeS. The present study was aimed to investigate the impact of chemerin rs17173608 and vaspin rs2236242 gene polymorphisms with the risk of MeS in a sample of Iranian population. This case-control study was done on 151 subjects with MeS and 149 without MeS, as defined by NCEP-ATPIII. Tetra amplification refractory mutation system-polymerase chain reaction (T-ARMS-PCR) was designed to detect the polymorphisms. Our finding showed that there are significant differences in genotype frequencies between the groups regarding vaspin rs2236242 polymorphism (χ(2)=18.74, p<0.0001). A significant protection against MeS was found for vaspin rs2236242 in allele and genotypes (Odd Ratio [OR]=0.52; 95% confidence interval [CI]=0.37-0.72; p=0.0001, T vs A; OR=0.49; 95%CI=0.29-0.82; p=0.007, TT vs TA and OR=0.17; 95%CI=0.07-0.40; p<0.0001, TT vs AA). Our finding showed positive association between chemerin rs17173608 polymorphism and risk of MeS (χ(2)=7.70, p=0.021). The G allele increased the risk of MeS (OR=1.78; 95% CI=1.14-2.78; p=0.012) as compared to the T allele. In conclusion, our data suggest for the first time a significant association between vaspin rs2236242 and chemerin rs17173608 polymorphisms and the MeS in Zahedan, southeast Iran. Further studies with large sample size and different ethnicities are required to confirm our findings.
代谢综合征（MeS）与 2 型糖尿病（T2DM）和心血管疾病的风险增加有关。有一些证据表明脂肪因子在 MeS 的发展中起作用。本研究旨在调查 chemerin rs17173608 和 vaspin rs2236242 基因多态性对伊朗人群中 MeS 风险的影响。这项病例对照研究对 151 名 MeS 患者和 149 名无 MeS 患者进行了研究，MeS 的定义是根据 NCEP-ATPIII。设计了四聚体扩增抗性突变系统-聚合酶链反应（T-ARMS-PCR）来检测多态性。我们的研究结果表明，在 vaspin rs2236242 多态性方面，两组之间的基因型频率存在显著差异（χ²=18.74，p<0.0001）。我们发现 vaspin rs2236242 等位基因和基因型对 MeS 有显著的保护作用（比值比[OR]=0.52；95%置信区间[CI]=0.37-0.72；p=0.0001，T 对 A；OR=0.49；95%CI=0.29-0.82；p=0.007，TT 对 TA 和 OR=0.17；95%CI=0.07-0.40；p<0.0001，TT 对 AA）。我们的研究结果表明，chemerin rs17173608 多态性与 MeS 风险之间存在正相关（χ²=7.70，p=0.021）。与 T 等位基因相比，G 等位基因增加了 MeS 的风险（OR=1.78；95%CI=1.14-2.78；p=0.012）。总之，我们的数据首次表明，伊朗东南部扎黑丹市 vaspin rs2236242 和 chemerin rs17173608 多态性与 MeS 之间存在显著关联。需要进一步进行更大样本量和不同种族的研究来证实我们的发现。
