Hasanvand Zahra, Sadeghi Abdorrahim, Rezvanfar Mohammad Reza, Goodarzi Mohammad Taghi, Rahmannezhad Golzar, Mashayekhi Farideh Jalali
Department of Clinical Biochemistry and Genetics, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran.
Department of Clinical Biochemistry and Genetics, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran; Molecular and Medicine Research Center, Arak University of Medical Sciences, Arak, Iran.
Gene. 2018 Apr 5;649:87-92. doi: 10.1016/j.gene.2018.01.061. Epub 2018 Jan 31.
Gestational diabetes mellitus (GDM) is defined as hyperglycemia detected during pregnancy and its risk is increased with obesity. Chemerin, an adipokine, has been proposed as potential mediators of insulin resistance in GDM. This case-control study was designed to assess the relation between chemerin SNPs rs4721 (or rs10278590) and rs17173608 and the development of GDM. One hundred thirty GDM pregnant women with GDM and 160 healthy pregnant women were enrolled in this study. The diagnosis of GDM was based on the International Association of Diabetes and Pregnancy Study Group (IADPSG) criteria. Chemerin rs4721 polymorphism gene was amplified through PCR, and SNP was detected using restriction enzyme AluI. Genotyping for chemerin rs17173608 polymorphism was performed by using tetra-amplification refractory mutation system polymerase chain reaction (T-ARMS-PCR). Blood glucose level was measured by an enzymatic method. Our finding showed that the genotypes frequency of chemerin rs4721 polymorphism was significantly different between GDM and non-GDM groups (χ = 7.44, P = 0.02). The genotype of rs4721 was significantly associated with GDM in co-dominant and dominant genotypes (GG vs GT, OR = 2.3, 95%CI = 1.24-4.24, P = 0.008, and GG vs GT + TT, OR = 2.21, 95%CI = 1.23-3.99, P = 0.008, respectively). No significant difference was observed in allele frequency between case and control groups (P = 0.62). Moreover, the genotypes and allele frequencies of chemerin rs17173608 polymorphism did not show significant differences between GDM and non-GDM (P > 0.05). We concluded that the genotype of rs4721 was found to contribute significant risk to GDM while genotype of rs17173608 could not predict the risk of GDM.
妊娠期糖尿病（GDM）被定义为在孕期检测到的高血糖，其风险会随着肥胖而增加。趋化素是一种脂肪因子，被认为是妊娠期糖尿病中胰岛素抵抗的潜在介质。本病例对照研究旨在评估趋化素单核苷酸多态性（SNP）rs4721（或rs10278590）和rs17173608与妊娠期糖尿病发生之间的关系。本研究纳入了130例患有妊娠期糖尿病的孕妇和160例健康孕妇。妊娠期糖尿病的诊断基于国际糖尿病与妊娠研究组（IADPSG）标准。通过聚合酶链反应（PCR）扩增趋化素rs4721多态性基因，并使用限制性内切酶AluI检测SNP。采用四引物扩增阻滞突变系统聚合酶链反应（T-ARMS-PCR）对趋化素rs17173608多态性进行基因分型。采用酶法测量血糖水平。我们的研究结果显示，趋化素rs4721多态性的基因型频率在妊娠期糖尿病组和非妊娠期糖尿病组之间存在显著差异（χ²=7.44，P=0.02）。在共显性和显性基因型中，rs4721的基因型与妊娠期糖尿病显著相关（GG与GT相比，OR=2.3，95%CI=1.24-4.24，P=0.008；GG与GT+TT相比，OR=2.21，95%CI=1.23-3.99，P=0.008）。病例组和对照组之间的等位基因频率没有显著差异（P=0.62）。此外，趋化素rs17173608多态性的基因型和等位基因频率在妊娠期糖尿病组和非妊娠期糖尿病组之间没有显著差异（P>0.05）。我们得出结论，发现rs4721的基因型对妊娠期糖尿病有显著风险，而rs17173608的基因型不能预测妊娠期糖尿病的风险。
